Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR.

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Abstract Text:

Lei Sun,Hongwei Gao,Vidya J Sarma,Ren-Feng Guo,Peter A Ward,

C5a, one of the most potent inflammatory peptides, induces its inflammatory functions by interacting with C5a receptor (C5aR) that belongs to the rhodopsin family of seven-transmembrane G protein-coupled receptors. C5a/C5aR signaling has been implicated in the pathogenesis of many inflammatory and immunological diseases such as sepsis and acute lung injury. Widespread upregulation of C5aR has been seen at both the protein level and transcriptional level under pathological conditions. Here, we show that C5aR gene expression can be specifically suppressed by siRNA, both in vitro and in vivo. A panel of chemically siRNA oligonucleotides was first synthesized to identify the functional siRNA sequences. The short hairpin RNAs (shRNAs) were also designed, cloned, and tested for the silencing effects in C5aR transfected cells. The effective shRNA expression cassettes were then transferred to an adenovirus DNA vector. ShRNA-expressing adenoviruses were intratracheally administered into mouse lung, and a significant in vivo silencing of C5aR was obtained four days after administration. Thus, C5aR shRNA-expressing adenoviruses appear to be an alternative strategy for the treatment of complement-induced disorders.

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Publishing Authors By Initials

L Sun,H Gao,VJ Sarma,RF Guo,PA Ward,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedicine & biotechnology

VOLUME: 2006

Page Numbers: 28945

Journal Abbreviation: J. Biomed. Biotechnol.

ISSN: 1110-7243

DAY: 23

MONTH: 10

YEAR: 2006

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101135740

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomed Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR Related Publications

• A model for the function of sperm DNA degradation.
• Adenovirus-Mediated In Vivo Silencing of Anaphylatoxin Receptor C5aR.
• Analyses of TCR clustering at the T cell-antigen-presenting cell interface and its impact on the activation of naive CD4+ T cells.
• Analysis of a Scottish founder effect narrows the TAPVR-1 gene interval to chromosome 4q12.
• Comparison of reproductive performance in Belgian dairy and beef cattle.
• Diagnosis of lymphogranuloma venereum from biopsy samples.
• Divergent activities of an engineered antibody in murine and human systems have implications for therapeutic antibodies.
• Early steps in the biosynthesis of MUC2 epithelial mucin in colon cancer cells.
• Feedback regulation of murine autoimmunity via dominant anti-inflammatory effects of interferon gamma.
• Heart sounds separation from lung sounds using independent component analysis.
• Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide.
• Immunization administration and disease reporting in the emergency department.
• In vitro methylation of specific regions in recombinant DNA constructs by excision and religation.
• In vivo regulation of neutrophil apoptosis by C5a during sepsis.
• Interstitial cells of Cajal: a new perspective on smooth muscle function.
• Is cycloxygenase-2 (COX-2) a major component of the mechanism responsible for microvascular remodeling in the brain?
• Kit mutants and gastrointestinal physiology.
• Kit signaling is essential for development and maintenance of interstitial cells of Cajal and electrical rhythmicity in the embryonic gastrointestinal tract.
• Measurement of perceived school climate for active travel in children.
• Microarray technology in biomedical research.
• Monoclonal antibodies reacting with the MUC2 mucin core protein.
• Pathology services. Down the tubes.
• Precancer in mice: animal models used to understand, prevent, and treat human precancers.
• Relationships between days post partum, observed estrus and uterine microflora in commercial dairy cows.
• Selectin inhibition modulates NF-kappa B and AP-1 signaling after liver ischemia/reperfusion.
• Simian varicella virus gene 61 encodes a viral transactivator but is non-essential for in vitro replication.
• Sperm chromatin remodeling after intracytoplasmic sperm injection differs from that of in vitro fertilization.
• The disconnect between animal models of sepsis and human sepsis.
• The influence of energy intake and percentage of body fat on the reproductive performance of nonpregnant mares.
• Topoisomerase IIB and an extracellular nuclease interact to digest sperm DNA in an apoptotic-like manner.