[Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells]

[Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells] Research Abstract Details 

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[Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells] Abstract Text:

Pei-li Yang,Yu-huan Liu,Ying Cui,Zai-long Cai,Ji-fang Mao,Pei-li Yang,Yu-huan Liu,Ying Cui,Zai-long Cai,Ji-fang Mao,

OBJECTIVE: To investigate whether the human endometrial carcinoma cells, RL95-2 infected with recombinant Ad-PTEN can steadily produce PTEN protein and enter apoptosis. METHODS: The recombinant adenovirus containing PTEN cDNA was constructed using the method of homologous recombination in bacteria. The viral titer was examined by plaque assay and the expression of PTEN protein was detected by western blot assay. The apoptosis of RL95-2 cells was evaluated as following: flipping of membrane phosphatidylserine (PS) and identification of activating caspase-3 positive cells was determined by flow cytometer (FCM), and furthermore genomic DNA fragmentation was detected by agarose electrophoresis. RESULTS: The recombinant adenovirus encoding PTEN cDNA was successfully constructed, and viral titers of Ad-PTEN were 5 x 10(9) pfu/ml. After infected by Ad-PTEN, the expression of PTEN protein was steady in human RL95-2 cells. After infected by Ad-PTEN for 24, 48, 72 and 96 h, the relative cell number of membrane PS flipping were (6.09 +/- 1.01)%, (9.98 +/- 2.17)%, (11.74 +/- 2.65)%, (27.69 +/- 8.67)%, which significantly increased than control group (P < 0.05), the relative cell number of activated caspase-3 positive were (2.6 +/- 0.5)%, (18.0 +/- 4.4)%, (21.8 +/- 5.1)%, (33.7 +/- 9.9)%, respectively, which significantly increased than control group (P < 0.05), and genomic DNA fragmentation was verified also. CONCLUSIONS: The recombinant Ad-PTEN vector is constructed successfully and the expression of specific PTEN is steady in RL95-2 cell line. The expression of PTEN induces RL95-2 cells to apoptosis. PTEN gene may be a novel therapeutic target in endometrial carcinoma.

[Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells] Publishing Authors By Initials

PL Yang,YH Liu,Y Cui,ZL Cai,JF Mao,PL Yang,YH Liu,Y Cui,ZL Cai,JF Mao,

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[Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua fu chan ke za zhi

VOLUME: 41

Page Numbers: 549-53

Journal Abbreviation: Zhonghua Fu Chan Ke Za Zhi

ISSN: 0529-567X

DAY: 6

MONTH: Aug

YEAR: 2006

[Adenoviral-mediated expression of PTEN cDNA induces apoptosis in endometrial carcinoma cells] Information

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LANGUAGE: chi

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AFFILIATION: Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

Country: China

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MEDLINETA: Zhonghua Fu Chan Ke Za Zhi

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