Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2.

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Abstract Text:

Marianne Demerjian,Jean-Pierre Hachem,Erwin Tschachler,Geertrui Denecker,Wim Declercq,Peter Vandenabeele,Theodora Mauro,Melanie Hupe,Debra Crumrine,Truus Roelandt,Evi Houben,Peter M Elias,Kenneth R Feingold,Marianne Demerjian,Jean-Pierre Hachem,Erwin Tschachler,Geertrui Denecker,Wim Declercq,Peter Vandenabeele,Theodora Mauro,Melanie Hupe,Debra Crumrine,Truus Roelandt,Evi Houben,Peter M Elias,Kenneth R Feingold,Marianne Demerjian,Jean-Pierre Hachem,Erwin Tschachler,Geertrui Denecker,Wim Declercq,Peter Vandenabeele,Theodora Mauro,Melanie Hupe,Debra Crumrine,Truus Roelandt,Evi Houben,Peter M Elias,Kenneth R Feingold,

Stratum corneum comprises corneocytes, derived from outer stratum granulosum during terminal differentiation, embedded in a lipid-enriched extracellular matrix, secreted from epidermal lamellar bodies. Permeability barrier insults stimulate rapid secretion of preformed lamellar bodies from the outer stratum granulosum, regulated through modulations in ionic gradients and serine protease (SP)/protease-activated receptor type 2 (PAR2) signaling. Because corneocytes are also required for barrier function, we hypothesized that corneocyte formation could also be regulated by barrier function. Barrier abrogation by two unrelated methods initiated a wave of cornification, assessed as TdT-mediated dUTP nick end-labeling-positive cells in stratum granulosum and newly cornified cells by electron microscopy. Because cornification was blocked by occlusion, corneocytes formed specifically in response to barrier, rather than injury or cell replacement, requirements. SP inhibitors and hyperacidification (which decreases SP activity) blocked cornification after barrier disruption. Similarly, cornification was delayed in PAR2(-/-) mice. Although classical markers of apoptosis [poly(ADP-ribose)polymerase and caspase (Casp)-3] remained unchanged, barrier disruption activated Casp-14. Moreover, the pan-Casp inhibitor Z-VAD-FMK delayed cornification, and corneocytes were structurally aberrant in Casp14(-/-) mice. Thus, permeability barrier requirements coordinately drive both the generation of the stratum corneum lipid-enriched extracellular matrix and the transformation of granular cells into corneocytes, in an SP- and Casp-14-dependent manner, signaled by PAR2.

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Publishing Authors By Initials

M Demerjian,JP Hachem,E Tschachler,G Denecker,W Declercq,P Vandenabeele,T Mauro,M Hupe,D Crumrine,T Roelandt,E Houben,PM Elias,KR Feingold,M Demerjian,JP Hachem,E Tschachler,G Denecker,W Declercq,P Vandenabeele,T Mauro,M Hupe,D Crumrine,T Roelandt,E Houben,PM Elias,KR Feingold,M Demerjian,JP Hachem,E Tschachler,G Denecker,W Declercq,P Vandenabeele,T Mauro,M Hupe,D Crumrine,T Roelandt,E Houben,PM Elias,KR Feingold,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The American journal of pathology

VOLUME: 172

Page Numbers: 86-97

Journal Abbreviation: Am. J. Pathol.

ISSN: 0002-9440

DAY: 21

MONTH: 12

YEAR: 2007

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370502

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2.

AFFILIATION: Dermatology Service (190), VA Medical Center, 4510 Clement St., San Francisco, CA 94121. eliasp@derm.ucsf.edu.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Pathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2 Related Publications

• A case of portal venous gas after extracorporeal shockwave lithotripsy and obstructive pyelonephritis.
• A hierarchical model for estimating significance levels of non-parametric linkage statistics for large pedigrees.
• Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2.
• Adverse drug interactions clinically important for the dermatologist.
• Antibiotic prophylaxis against bacterial endocarditis in patients undergoing dermatologic surgery.
• Bacterial adherence, colonization, and pathogenicity.
• Cell Shape Dynamics in Escherichia coli.
• Deficiency of PPARbeta/delta in the Epidermis Results in Defective Cutaneous Permeability Barrier Homeostasis and Increased Inflammation.
• Does the tail wag the dog? Role of the barrier in the pathogenesis of inflammatory dermatoses and therapeutic implications.
• Expression of the phospholipid scramblase (PLSCR) gene family during the acute phase response.
• Gene-dropping vs. empirical variance estimation for allele-sharing linkage statistics.
• Glucocorticoid blockade reverses psychological stress-induced abnormalities in epidermal structure and function.
• In vivo and in vitro regulation of sterol 27-hydroxylase in the liver during the acute phase response. potential role of hepatocyte nuclear factor-1.
• Medical and vocational cooperation for the aging.
• PPAR and LXR activators regulate ABCA12 expression in human keratinocytes.
• Patterns of expressed emotion and patient coping styles that characterise the families of recent onset schizophrenics.
• Principles of human genetics in relation to insurance medicine and public health.
• Random thoughts continued.
• Regulation of ABCA1 expression in human keratinocytes and murine epidermis.
• Robust Score Statistics for QTL Linkage Analysis.
• Segregation analysis of the Jacobsen data.
• Staphylococcal scalded skin syndrome in an adult.
• Subtyping familial schizophrenia: reliability, concordance, and stability.
• The importance of lipids in cutaneous function.
• The influence of acute infection upon the course of allergy in children; some clinical observations.
• Thematic Review Series: Skin Lipids. Peroxisome proliferator-activated receptors and liver X receptors in epidermal biology.
• Thematic review series: Skin Lipids. The role of epidermal lipids in cutaneous permeability barrier homeostasis.
• Tumor necrosis factor and interleukin 1 decrease RXRalpha, PPARalpha, PPARgamma, LXRalpha, and the coactivators SRC-1, PGC-1alpha, and PGC-1beta in liver cells.
• Tumor necrosis factor, cytokines, and the hyperlipidemia of infection.
• Web-Based Identification of Evolutionary Conserved DNA cis-Regulatory Elements.