Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method.

Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method. Research Abstract Details 

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Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method. Abstract Text:

Masafumi Uematsu,Takeshi Kumagami,Mao Kusano,Koki Yamada,Kazuaki Mishima,Koichi Fujimura,Hitoshi Sasaki,Takashi Kitaoka,Masafumi Uematsu,Takeshi Kumagami,Mao Kusano,Koki Yamada,Kazuaki Mishima,Koichi Fujimura,Hitoshi Sasaki,Takashi Kitaoka,Masafumi Uematsu,Takeshi Kumagami,Mao Kusano,Koki Yamada,Kazuaki Mishima,Koichi Fujimura,Hitoshi Sasaki,Takashi Kitaoka,

OBJECTIVE: Acute corneal permeability change after instillation of benzalkonium chloride (BAC) was evaluated using a newly developed in vivo corneal transepithelial electric resistance (TER) measurement method. METHOD: Corneal TER was measured by Ag/AgCl electrodes placed in the anterior aqueous chamber and on the cornea of live rabbit eyes. TER was measured and TER change after instillation of 0.05% BAC solution was monitored. After TER measurement, cornea was excised and fixed for transmission and scanning electron microscopy. For the control study, physiologic saline was used instead of BAC. RESULTS: The TER of normal rabbit cornea was 602.3 +/- 195.0 Omega cm(2). TER decreased instantly after instillation of 0.05% BAC. In 5 s, TER decreased to 58.3 +/- 5.2%. In 60 s, TER decreased to 18.5 +/- 3.2%. At all time points, TER after instillation of 0.05% BAC was significantly lower than that of the control (p < 0.0001). Dissociation of tight junctions and the destruction of superficial cell membranes were observed under electron microscopy. CONCLUSION: Corneal epithelial change with increased permeability is rapid and intense after the instillation of highly concentrated BAC solution, accompanied by disorder of tight junctions and cell membranes of superficial cells. The newly developed in vivo corneal TER measurement method is suitable for assessing acute corneal change after drug instillation.

Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method. Publishing Authors By Initials

M Uematsu,T Kumagami,M Kusano,K Yamada,K Mishima,K Fujimura,H Sasaki,T Kitaoka,M Uematsu,T Kumagami,M Kusano,K Yamada,K Mishima,K Fujimura,H Sasaki,T Kitaoka,M Uematsu,T Kumagami,M Kusano,K Yamada,K Mishima,K Fujimura,H Sasaki,T Kitaoka,

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Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Ophthalmic research

VOLUME: 39

Page Numbers: 308-14

Journal Abbreviation: Ophthalmic Res.

ISSN: 1423-0259

DAY: 22

MONTH: 10

YEAR: 2007

Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method. Information

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LANGUAGE: eng

NlmUniqueID: 267442

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Grant and Affiliation Information for Acute corneal epithelial change after instillation of benzalkonium chloride evaluated using a newly developed in vivo corneal transepithelial electric resistance measurement method.

AFFILIATION: Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan. uematsu-ngs@umin.ac.jp

Country: Switzerland

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MEDLINETA: Ophthalmic Res

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