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Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles.

Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Research Abstract Details 

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  • Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Abstract Text:

    ansil dyalAnsil Dyal,katja loosKatja Loos,mayumi notoMayumi Noto,seung w changSeung W Chang,chiara spagnoliChiara Spagnoli,kurikka v p m shafiKurikka V P M Shafi,abraham ulmanAbraham Ulman,mary cowmanMary Cowman,richard a grossRichard A Gross,

    We report the stability and enzymatic activity of Candida rugosa Lipase (E.C.3.1.1.3) immobilized on gamma-Fe2O3 magnetic nanoparticles. The immobilization strategies were either reacting the enzyme amine group with a nanoparticle surface acetyl, or amine groups. In the former, the enzyme was attached through a C=N bond, while in the latter it was connected using glutaraldehyde. AFM images show an average particle size of 20 +/- 10 nm after deconvolution. The enzymatic activity of the immobilized lipase was determined by following the ester cleavage of p-nitrophenol butyrate. The covalently immobilized enzyme was stabile and reactive over 30 days.

    Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Publishing Authors By Initials

    a dyalA Dyal,k loosK Loos,m notoM Noto,sw changSW Chang,c spagnoliC Spagnoli,kv shafiKV Shafi,a ulmanA Ulman,m cowmanM Cowman,ra grossRA Gross,

    For similar natural sciences: nanotechnology research abstracts see: natural sciences: nanotechnology research

    PUBMED ID PMID:

    MEDLINE DATE:

    Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Journal Published:

    PUBLICATION TYPE: Research Support, U.S. Gov't,

    Journal: Journal of the American Chemical Society

    VOLUME: 125

    Page Numbers: 1684-5

    Journal Abbreviation: J. Am. Chem. Soc.

    ISSN: 0002-7863

    DAY: 19

    MONTH: Feb

    YEAR: 2003

    Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 7503056

    Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Keywords Mesh Terms:

    KEYWORDS: Nanotechnology

    MESH TERMS: methods

    Chemical & Substance for Abstract: Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles. Information

    Substance Name: Lipase

    Registry Number: EC 3.1.1.3

    Grant and Affiliation Information for Activity of Candida rugosa lipase immobilized on gamma-Fe2O3 magnetic nanoparticles.

    AFFILIATION: Department of Chemical Engineering, Chemistry and Material Science, Polytechnic University, 6 Metrotech Center, Brooklyn, New York 11201, USA.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: J Am Chem Soc

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