Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts.

Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Research Abstract Details 

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Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Abstract Text:

Ulrich ,Richard Tuli,Reza Seghatoleslami,Michael Howard,Asit Shah,David J Hall,Noreen J Hickok,Rocky S Tuan,

The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported that BMP-2 treatment increase cytoskeletal organization of human trabecular bone-derived osteoblast-like cells (osteoblasts), which is also accompanied by an activation of the focal adhesion kinase p125(FAK). We report here that activation of p125(FAK) occurs with the same kinetics as the phosphorylation of Smad1, suggesting that BMP-2 initiates cross-talk between Smad signaling and the adhesion-mediated signaling pathway. As an adjunct to these effects, we examined activation of mitogen-activated protein (MAP) kinase family members in response to focal adhesion contact formation. Although phosphorylated forms of all three kinases were apparent, only SAPK2alpha/p38 (p38) was activated in response to BMP-2 treatment. Inhibition of p38 kinase activity suppressed BMP-2 induced Smad1 phosphorylation, as well as its translocation to the nucleus, suggesting the integration of p38 activation with Smad1 signaling. Finally, inhibition of p38 in osteoblasts also led to the complete abrogation of BMP-2 induced osteocalcin gene expression and matrix mineralization. These findings suggest that BMP-2 must activate p38 in order to mediate osteogenic differentiation and maturation.

Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Publishing Authors By Initials

U ,R Tuli,R Seghatoleslami,M Howard,A Shah,DJ Hall,NJ Hickok,RS Tuan,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research

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Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Experimental cell research

VOLUME: 291

Page Numbers: 201-11

Journal Abbreviation: Exp. Cell Res.

ISSN: 0014-4827

DAY: 15

MONTH: Nov

YEAR: 2003

Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 373226

Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Keywords Mesh Terms:

KEYWORDS: p38 Mitogen-Activated Protein Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts. Information

Substance Name: p38 Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts.

AFFILIATION: Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: DE 16865

ACRONYM: DE

MEDLINETA: Exp Cell Res

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