Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons.

Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Research Abstract Details 

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Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Abstract Text:

Ken Ito,Shinichi Iwasaki,Kenji Kondo,Didier Dulon,Kimitaka Kaga,

In a previous study we showed that, in rat spiral ganglion neurons (SGNs), the adenosine 5'-triphosphate (ATP)-evoked currents were a combination of the activation of ionotropic receptors (the first fast current) and the activation of metabotropic receptors which secondarily opened non-selective cation channels. These two conductances imply the involvement of different receptor subtypes. In the present study, we tested three subtype-specific purinergic ligands: alpha,beta-methylene ATP (a;pha,beta-meATP) for P2X receptors, uridine 5'-triphosphate (UTP) for P2Y receptors and 2'-3'-O-(4-benzoylbenzoyl) ATP (Bz-ATP) for P2Z (P2X(7)) receptors. Application of 100 microM alpha,beta-meATP did not trigger any significant change in membrane conductance, while the SGNs were responsive to ATP. Pressure application of UTP (100 microM, 1 s) evoked an inward current averaging 344+/-169 pA at a holding potential of -50 mV. The conductance developed after a latency averaging 1.5+/-0.6 s, took 4-6 s to peak and reversed slowly within 15-30 s. The current-voltage curve reversed near 0 mV, suggesting a non-selective cation conductance, like the second component of the ATP conductance. Bz-ATP evoked an inward current which developed without latency, was sustained during ligand application and was rapidly inactivated at the end of application: the same characteristics as the first component of the ATP-evoked current. The Bz-ATP conductance reversed around -10 mV, indicating also a non-selective cation conductance. These results suggest that, in SGNs, ATP acts via two different receptor subtypes, ionotropic P2Z receptors and metabotropic P2Y receptors, and that these two receptor subtypes can assume different physiological roles.

Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Publishing Authors By Initials

K Ito,S Iwasaki,K Kondo,D Dulon,K Kaga,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: uracil nucleotides: uridine triphosphate research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: uracil nucleotides: uridine triphosphate research

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Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta oto-laryngologica. Supplementum

VOLUME:

Page Numbers: 23-7

Journal Abbreviation:

ISSN: 0365-5237

DAY: 13

MONTH: Aug

YEAR: 2004

Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370355

Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Keywords Mesh Terms:

KEYWORDS: Uridine Triphosphate

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons. Information

Substance Name: 3'-O-(4-benzoyl)benzoyladenosine 5'-trip

Registry Number: 81790-82-1

Grant and Affiliation Information for Actions of subtype-specific purinergic ligands on rat spiral ganglion neurons.

AFFILIATION: Department of Otolaryngology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. itoken-tky@umin.ac.jp

Country: Norway

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MEDLINETA: Acta Otolaryngol Suppl

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