Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator.

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Abstract Text:

Clomiphene citrate (CC), a synthetic oestrogen, is often prescribed as a superovulator in treating infertility. Although CC works efficiently, pregnancy rates following CC treatment are approximately 10 times lower than "natural" rates. This study investigates how a dose of 1.25mg CC given to ovariectomized rats before the implantation priming hormones (a single dose of progesterone for 3 days and a dose of estrodiol-17beta on d3, P-P-PE), alters the expression and distribution of alpha-actinin, gelsolin and vinculin. Actin binding proteins show a specific distribution within the uterine epithelium during implantation, linking the actin cytoskeleton to integrin expression on the uterine surface and in this way aiding "adhesiveness" for blastocyst apposition to the uterine epithelium. In this study, immunocytochemistry on frozen uterine sections using mouse monoclonal antibodies against alpha-actinin, gelsolin and vinculin and peroxidase-conjugated secondary antibodies, show that CC, administered before the P-P-PE regimen, down-regulates the expression of vinculin, does not alter the expression of gelsolin and up-regulates alpha-actinin on the uterine apical surface, when compared to P-P-PE treated animals. All three proteins are down-regulated on the apical surface of the luminal epithelium and glands in all groups when compared to pregnant controls. Vinculin was only localized in the basolateral compartment of the uterine epithelial cells in the CC treated groups. By down-regulating these proteins on the uterine surface and up-regulating vinculin on the basolateral membrane of the epithelium, CC may impede adhesion and invasion of blastocysts at implantation. These results may aid the exogenous manipulation of uterine tissue to control fertility and improve assisted reproductive out-comes.

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 69

Page Numbers: 700-13

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 6

MONTH: 02

YEAR: 2008

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 421510

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator.

AFFILIATION: School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road Parktown, Gauteng, 2193 Johannesburg, South Africa.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Theriogenology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator Related Publications

• A histopathologic evaluation of the plasma skin regeneration system (PSR) versus a standard carbon dioxide resurfacing laser in an animal model.
• Actin binding protein expression is altered in uterine luminal epithelium by clomiphene citrate, a synthetic estrogen receptor modulator.
• Airborne estimation of sea turbidity parameters from the WRELADS laser airborne depth sounder.
• Alpha 1 antitrypsin serum levels and phenotypes in patients with retinal vasculitis.
• Application of a constrained external fixator frame for treatment of a fixed equinus contracture.
• Bayesian M/EEG source reconstruction with spatio-temporal priors.
• Bird evolution: testing the Metaves clade with six new mitochondrial genomes.
• Blood hyperviscosity with reduced skin blood flow in scleroderma.
• Conservative management or closed nailing for tibial shaft fractures. A randomised prospective trial.
• Cradles through the ages.
• Cytomegalovirus infection in a physician.
• Epidemic influenza in schools.
• Epidemiology of gastrointestinal nematode parasitism in a commercial sheep flock and its implications for control programmes.
• Excessive production of mucoid substance in staphylococcus SIIA: a possible factor in colonisation of Holter shunts.
• Familial occurrence of alpha 1-antitrypsin deficiency and Weber-Christian disease.
• Feline truncal ataxia associated with degeneration of the cerebellar cortex and roof nuclei.
• Formulation and statistical optimization of a novel crosslinked polymeric anti-tuberculosis drug delivery system.
• Immune complexes and human disease.
• Immunological abnormalities in patients with untreated retinal vasculitis.
• Letter: Extrinsic allergic alveolitis.
• Management of the sick neonate with suspected heart disease.
• Modeling depth bias in an airborne laser hydrographic system.
• Population-based study of antenatal detection of congenital heart disease by ultrasound examination.
• Prescribing patterns of gerontological nurse practitioners in the United States.
• Sensitivity to eye gaze in autism: Is it normal? Is it automatic? Is it social?
• Simultaneous pulmonary trunk and pulmonary arterial wave intensity analysis in fetal lambs: evidence for cyclical, midsystolic pulmonary vasoconstriction.
• The Mekong at climatic crossroads: Lessons from the geological past.
• The role of the duodenal microflora as a determinant of persistent diarrhoea.
• Umbilical hernia and abdominal cramp in swimmers.
• Unusual cause of persistent impairment of ventricular function after repair of coarctation of the aorta.