Acoustics of blood plasma on solid surfaces.

Acoustics of blood plasma on solid surfaces. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Acoustics of blood plasma on solid surfaces. Abstract Text:

Marcus Andersson,Anders Sellborn,Camilla Fant,Christina Gretzer,Hans Elwing,

We have quantified surface associated coagulation of human blood plasma with a recently developed methodological system consisting of a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), a method that measures the weight of adsorbed molecules on surfaces as a function of frequency shifts of a quartz crystal. Further, it measures the damping energy (i.e. viscoelasticity) of the adsorbed layer. Four different surfaces where studied: Heparin (Hep) surface as an active inhibitor of clot formation, titanium (Ti) surfaces that are known to activate the intrinsic pathway, polystyrene (PS) surfaces and poly(urethane urea) (PUUR) surfaces. The experiments were initiated by applying citrated human plasma at the sensor surfaces; calcium was then added toinitiate coagulation. The Hep surfaces showed no apparent indication of clot formation during one hour of incubation at room temperature. However, on Ti surfaces we observed an early and rapid change in both frequency shift and viscoelastic properties of the coagulating plasma. We inhibited the intrinsic pathway activation by using corn trypsin inhibitor (CTI), which is specific for factor FXIIa in the bulk phase, which prolonged the coagulation times for all non-heparinized surfaces. We have also found a peculiar initial plasma protein interaction phenomenon on Ti surfaces. The described methodology would be very efficient for basic studies of surface associated coagulation and as a screening method for new biomaterials.

Acoustics of blood plasma on solid surfaces. Publishing Authors By Initials

M Andersson,A Sellborn,C Fant,C Gretzer,H Elwing,

For similar natural sciences: weights and measures research abstracts see: natural sciences: weights and measures research

PUBMED ID PMID:

MEDLINE DATE:

Acoustics of blood plasma on solid surfaces. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 13

Page Numbers: 907-17

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2002

Acoustics of blood plasma on solid surfaces. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Acoustics of blood plasma on solid surfaces. Keywords Mesh Terms:

KEYWORDS: Weights and Measures

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Acoustics of blood plasma on solid surfaces. Information

Substance Name: Mitrathane

Registry Number: 97343-15-2

Grant and Affiliation Information for Acoustics of blood plasma on solid surfaces.

AFFILIATION: Department of Cell and Molecular Biology/Interface biophysics, Lundberg Laboratory, Göteborg University, Box 462, SE-405 30 Göteborg, Sweden.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomater Sci Polym Ed

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Acoustics of blood plasma on solid surfaces Related Publications

• Acoustics of blood plasma on solid surfaces.
• Adsorption of fibrinogen and some other proteins from blood plasma at a variety of solid surfaces.
• Quartz crystal microbalance-with dissipation monitoring (QCM-D) for real time measurements of blood coagulation density and immune complement activation on artificial surfaces.
• The chemiluminescence response of neutrophils on polymer surfaces made by glow discharge plasma polymerization.
• The interaction between model biomaterial coatings and nylon microparticles as measured with a quartz crystal microbalance with dissipation monitoring.