Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity.

Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Research Abstract Details 

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Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Abstract Text:

We recently showed that acidosis is protective during hypoxia and detrimental during reoxygenation. We hypothesized that the detrimental effect of acidosis during reoxygenation was due to a negative effect on mitochondrial function. Human postmitotic NT2-N neurons were exposed to 3 h of hypoxia and glucose deprivation and then reoxygenated for 0, 1, 4, 9, or 21 h. The detrimental effect of acidotic reoxygenation on metabolic activity was evident already after 1 h of reoxygenation, when MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] reduction (percentage of normoxic controls) was significantly higher in cells reoxygenated with neutral compared with acidotic medium both after acidotic hypoxia (83+/-26% versus 67+/-27%, p=0.006) and after neutral hypoxia (51+/-12% versus 41+/-7%, p=0.005). Hypoxanthine, a marker of cellular energy failure, increased more with acidotic compared with neutral reoxygenation both after acidotic hypoxia (after 21 h: 7.7+/-2.7 versus 3.1+/-1.9 microM, p<0.001) and after neutral hypoxia (10.4+/-2.6 versus 7.9+/-2.8 microM, p=0.001). During hypoxia and reoxygenation, there was an earlier reduction in the activity of complex IV compared with complexes II+III, and the ratio between these complexes fell during the first hour of reoxygenation. The reduction in complex IV activity was alleviated with acidotic hypoxia. Acidosis during reoxygenation, however, had no effect on the activity of either complex IV or complexes II+III. We conclude that acidosis during hypoxia increases neuronal survival and preserves complex IV activity. Acidosis during reoxygenation has an early detrimental effect on metabolic activity, but this is not mediated through an effect on the mitochondrial complexes IV or II+III.

Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Publishing Authors By Initials

For similar surgical procedures, operative: cardiovascular surgical procedures: reperfusion research abstracts see: surgical procedures, operative: cardiovascular surgical procedures: reperfusion research

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Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Pediatric research

VOLUME: 57

Page Numbers: 488-93

Journal Abbreviation: Pediatr. Res.

ISSN: 0031-3998

DAY: 4

MONTH: 02

YEAR: 2005

Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Information

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LANGUAGE: eng

NlmUniqueID: 100714

Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Keywords Mesh Terms:

KEYWORDS: Reperfusion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity. Information

Substance Name: Oxygen

Registry Number: 7782-44-7

Grant and Affiliation Information for Acidosis during reoxygenation has an early detrimental effect on neuronal metabolic activity.

AFFILIATION: Department of Pediatric Research, Rikshospitalet University Clinic, Oslo, Norway. elisabeth.frøyland@medisin.uio.no

Country: United States

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MEDLINETA: Pediatr Res

REFSOURCE: Pediatr Res. 2008 Mar;63(3):314

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