Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica.

Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Research Abstract Details 

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Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Abstract Text:

J Christian Perez,Eduardo A Groisman,

Acid pH often triggers changes in gene expression. However, little is known about the identity of the gene products that sense fluctuations in extracytoplasmic pH. The Gram-negative pathogen Salmonella enterica serovar Typhimurium experiences a number of acidic environments both inside and outside animal hosts. Growth in mild acid (pH 5.8) promotes transcription of genes activated by the response regulator PmrA, but the signalling pathway(s) that mediates this response has thus far remained unexplored. Here we report that this activation requires both PmrA's cognate sensor kinase PmrB, which had been previously shown to respond to Fe(3+) and Al(3+), and PmrA's post-translational activator PmrD. Substitution of a conserved histidine or of either one of four conserved glutamic acid residues in the periplasmic domain of PmrB severely decreased or abolished the mild acid-promoted transcription of PmrA-activated genes. The PmrA/PmrB system controls lipopolysaccharide modifications mediating resistance to the antibiotic polymyxin B. Wild-type Salmonella grown at pH 5.8 were > 100 000-fold more resistant to polymyxin B than organisms grown at pH 7.7. Our results suggest that protonation of the PmrB periplasmic histidine and/or of the glutamic acid residues activate the PmrA protein, and that mild acid promotes cellular changes resulting in polymyxin B resistance.

Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Publishing Authors By Initials

JC Perez,EA Groisman,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular microbiology

VOLUME: 63

Page Numbers: 283-93

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 3

MONTH: Jan

YEAR: 2007

Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica. Information

Substance Name: pmrA protein, Bacteria

Registry Number: 0

Grant and Affiliation Information for Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica.

AFFILIATION: Program in Molecular Genetics, Howard Hughes Medical Institute, Washington University School of Medicine, Campus Box 8230, 660 S. Euclid Ave., St Louis, MO 63110, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI42336

ACRONYM: AI

MEDLINETA: Mol Microbiol

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