Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells.

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Abstract Text:

OBJECTIVES: For patients with Barrett's esophagus, physicians commonly prescribe antisecretory medications in dosages above those required to heal reflux esophagitis because acid has been shown to have proproliferative and antiapoptotic effects on Barrett's cancer cells and on Barrett's mucosal explants. For a number of reasons, these model systems may not be ideal for determining the effects of acid on benign Barrett's epithelial cells, however. We studied the effects of acid on proliferation and apoptosis in a nonneoplastic, telomerase-immortalized Barrett's epithelial cell line. METHODS: Barrett's cells were treated with two 3-minute exposures to acidic media. Cell growth was determined using cell counts, proliferation was studied by flow cytometry, cell viability was determined by trypan blue staining, and apoptosis was assessed by TUNEL and Annexin V. The expression levels of p53 and p21 were determined by Western blotting. p53 siRNA was used to study the effect of p53 inhibition on total cell numbers after acid exposure. RESULTS: Acid exposure significantly decreased total cell numbers at 24 h without affecting either cell viability or apoptosis. Acid exposure resulted in cell cycle prolongation that was associated with greater expression of p53, but not p21. The acid-induced decrease in total cell numbers was abolished by p53 RNAi. CONCLUSIONS: Acid exposure has p53-mediated, antiproliferative effects in nonneoplastic Barrett's epithelial cells. These findings contradict the results of prior in vitro and ex vivo studies. We speculate that the prescription of antisecretory medications in dosages beyond those required to heal gastroesophageal reflux disease (GERD) symptoms and endoscopic signs could be detrimental. Controlled, prospective clinical trials are needed to determine the optimal level of acid suppression for patients with Barrett's esophagus.

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Publishing Authors By Initials

For similar environment and public health: environment: meteorological factors: atmosphere: weather: sunlight: ultraviolet rays research abstracts see: environment and public health: environment: meteorological factors: atmosphere: weather: sunlight: ultraviolet rays research

PUBMED ID PMID:

MEDLINE DATE:

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The American journal of gastroenterology

VOLUME: 102

Page Numbers: 10-20

Journal Abbreviation: Am. J. Gastroenterol.

ISSN: 0002-9270

DAY: 3

MONTH: Jan

YEAR: 2007

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 421030

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Keywords Mesh Terms:

KEYWORDS: Ultraviolet Rays

MESH TERMS: metabolism

Chemical & Substance for Abstract: Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells. Information

Substance Name: Hydrochloric Acid

Registry Number: 7647-01-0

Grant and Affiliation Information for Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells.

AFFILIATION: Department of Medicine, Dallas VA Medical Center, University of Texas-Southwestern Medical School, Dallas, Texas 75216, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL67256

ACRONYM: HL

MEDLINETA: Am J Gastroenterol

REFSOURCE: Am J Gastroenterol. 2007 Jan;102(1):21-3

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells Related Publications

• A Programmable System of Functional Electrical Stimulation (FES).
• A typical presentation of cutaneous leishmaniasis after renal transplantation.
• Acid has antiproliferative effects in nonneoplastic Barrett's epithelial cells.
• All trans-retinoic acid induces apoptosis via p38 and caspase pathways in metaplastic Barrett's cells.
• Assessment of Acute Skin Irritation in Rabbits using Electrical Impedance Model.
• Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T).
• Dimensional crossover in the purple bronze Li0.9Mo6O17.
• Granular fingers on jammed systems: new fluidlike patterns arising in grain-grain invasion experiments.
• In non-neoplastic Barrett's epithelial cells, acid exerts early antiproliferative effects through activation of the Chk2 pathway.
• Inhibition of rats extramedullary liver erytropoiesis by hyperbaric oxygen therapy.
• Longitudinal studies of inter-alpha inhibitor proteins in severely septic patients: a potential clinical marker and mediator of severe sepsis.
• Naturally occurring compounds affect glutamatergic neurotransmission in rat brain.
• Novel cyclopalladated and coordination palladium and platinum complexes derived from alpha-diphenyl ethanedione bis(thiosemicarbazones): structural studies and cytotoxic activity against human A2780 and A2780cisR carcinoma cell lines.
• Proposal of a guanine-based purinergic system in the mammalian central nervous system.
• Quinolinic Acid-induced Seizures Stimulate Glutamate Uptake into Synaptic Vesicles from Rat Brain: Effects Prevented by Guanine-based Purines.
• Silicoproteinosis: high-resolution CT findings in 13 patients.
• Simplified Distributed-Parameter model of Brachial-Radial Arteries for Noninvasive Determination of Mechanical Characteristics of Vessel.
• Temporal envelope changes of compression and speech rate: combined effects on recognition for older adults.
• Ternary complex formation between AmtB, GlnZ and the nitrogenase regulatory enzyme DraG reveals a novel facet of nitrogen regulation in bacteria.
• The role of macrophage migration inhibitory factor in the cascade of events leading to reperfusion-induced inflammatory injury and lethality.
• Thoughts on the complex relationship between gastroesophageal reflux disease and eosinophilic esophagitis.
• [Clinical and morphometric evaluations of the angiogenic capacity of chitosan membrane in rabbit corneas]
• [Comparative study of several sinusitis experimental modelling techniques in rabbits.]
• [Impact on tuberculosis incidence rates of removal of repeat notification records.]
• [Mortality trends due to tuberculosis in Brazil, 1980-2004.]
• [Ophthalmologic conditions of AIDS patients with long-term follow-up]
• [Psychotic syndrome developing into dementia as a clinical manifestation of mitochondrial DNA deletion]
• [Surgical removal of a posttraumatic intracardiac foreign body in a child.]
• [Transient diabetes mellitus related to L-asparaginase therapy]
• [Tuberculosis incidence and cure rates, Brazil, 2000-2004.]