Accumulation of exogenous phosphoenolpyruvate against the concentration gradient was observed when human red cells were incubated in an acidified isotonic sucrose medium. Fluoride increased the apparent accumulation by inhibition of the intracellular metabolic interconversion of the phosphate compound. The accumulation appeared to be specific for phosphoenolpyruvate and the accumulation rate for 3-phosphoglycerate, which has a molecular size and pKa similar to those of phosphoenolpyruvate, was less than one-tenth of the rate of phosphoenolpyruvate. Red cells incubated in the acidified sucrose medium tended to adhere to each other when examined with a scanning electron microscope.
Accumulation of phosphoenolpyruvate in red cells incubated with the phosphate ester in an acidified isotonic sucrose medium. Publishing Authors By Initials
Accumulation of phosphoenolpyruvate in red cells incubated with the phosphate ester in an acidified isotonic sucrose medium. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 81
Page Numbers: 1505-9
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: May
YEAR: 1977
Accumulation of phosphoenolpyruvate in red cells incubated with the phosphate ester in an acidified isotonic sucrose medium. Information
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LANGUAGE: eng
NlmUniqueID: 376600
Accumulation of phosphoenolpyruvate in red cells incubated with the phosphate ester in an acidified isotonic sucrose medium. Keywords Mesh Terms:
KEYWORDS: Sucrose
MESH TERMS: pharmacology
Chemical & Substance for Abstract: Accumulation of phosphoenolpyruvate in red cells incubated with the phosphate ester in an acidified isotonic sucrose medium. Information
Substance Name: Phosphoenolpyruvate
Registry Number: 73-89-2
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Country: JAPAN
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MEDLINETA: J Biochem
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