Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro.

Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Research Abstract Details 

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Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Abstract Text:

Markus Herrmann,Natalia Umanskaya,Britt Wildemann,Graziana Colaianni,Johannes Schmidt,Thomas Widmann,Alberta Zallone,Wolfgang Herrmann,

BACKGROUND: Homocysteine (HCY) has recently been linked to fragility fractures. Elevated circulating HCY is mainly caused by folate, vitamin B12 and B6 deficiencies. However, little is known about the effect of these vitamins on the activity of osteoblasts. We hypothesized that decreasing concentrations of folate, vitamin B12 and B6 decrease osteoblasts activity by accumulation of HCY. METHODS: Osteoblasts obtained from trabecular human bone specimens of 8 donors were cultured with decreasing concentrations of folate, vitamin B12 and B6. Vitamin concentrations were modified in combination or one vitamin only (8 repetitions x 8 donors, n=64). After 14 days alkaline phosphatase (AP) activity, pro-collagen type I N-terminal peptide (PINP) and osteocalcin secretion in the supernatant was measured. After 20 days, the formation of mineralized matrix was analyzed. RESULTS: Decreasing B-vitamin concentrations induced a significant accumulation of HCY in the supernatant reaching up to 160%. The increase in HCY was not accompanied by changes of AP, osteocalcin and PINP. Moreover, mineralized matrix formation was not affected. CONCLUSION: Accumulation of HCY by decreasing concentrations of folate, vitamin B12 and B6 does not affect the activity of human osteoblasts. Consequently, other mechanisms have to be responsible for the reduced bone quality in hyperhomocysteinemic subjects.

Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Publishing Authors By Initials

M Herrmann,N Umanskaya,B Wildemann,G Colaianni,J Schmidt,T Widmann,A Zallone,W Herrmann,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyridines: picolines: vitamin b 6 research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyridines: picolines: vitamin b 6 research

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Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Clinica chimica acta; international journal of cli

VOLUME: 384

Page Numbers: 129-34

Journal Abbreviation: Clin. Chim. Acta

ISSN: 0009-8981

DAY: 4

MONTH: 07

YEAR: 2007

Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 1302422

Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Keywords Mesh Terms:

KEYWORDS: Vitamin B 6

MESH TERMS: metabolism

Chemical & Substance for Abstract: Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro. Information

Substance Name: Vitamin B 6

Registry Number: 8059-24-3

Grant and Affiliation Information for Accumulation of homocysteine by decreasing concentrations of folate, vitamin B12 and B6 does not influence the activity of human osteoblasts in vitro.

AFFILIATION: Department of Clinical Chemistry and Laboratory Medicine, University Hospital of Saarland, D-66421 Homburg/Saar, Germany. markusherr@aol.com

Country: Netherlands

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MEDLINETA: Clin Chim Acta

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