Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice.

Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Research Abstract Details 

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Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Abstract Text:

Sanjay K Jain,S Moises Hernandez-Abanto,Qi-Jian Cheng,Prabhpreet Singh,Lan H Ly,Lee G Klinkenberg,Norman E Morrison,Paul J Converse,Eric Nuermberger,Jacques Grosset,David N McMurray,Petros C Karakousis,Gyanu Lamichhane,William R Bishai,

BACKGROUND: Mouse and guinea pig models have been used to identify Mycobacterium tuberculosis mutants attenuated for survival. However, unlike mice, M. tuberculosis-infected guinea pigs form caseating granulomas, which may simulate human disease more closely. METHODS: We used designer arrays for defined mutant analysis, a high-throughput subtractive competition assay, for genotypically defined M. tuberculosis mutants and compared the survival of the same mutant pools in guinea pig and mouse aerosol models. Selected mutants found to be attenuated in either aerosol model were also analyzed in the mouse hollow-fiber model. RESULTS: M. tuberculosis mutants representing 74 genes were tested. Eighteen M. tuberculosis mutants were attenuated for survival in either aerosol model, with 70% of selected mutants also attenuated in the mouse hollow-fiber model. The majority of attenuated mutants in the mouse aerosol model were detected only after 90 days of infection. There was a high degree of concordance between the genes identified by the 2 aerosol models, with detection being significantly earlier in the guinea pig (P<.0003). CONCLUSIONS: We identified M. tuberculosis genes required for survival in mammalian lungs. The majority of mouse late-stage survival mutants were detected significantly earlier in the guinea pig, which suggests that differences in tuberculosis-induced lung pathologic changes may account for this accelerated detection.

Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Publishing Authors By Initials

SK Jain,SM Hernandez-Abanto,QJ Cheng,P Singh,LH Ly,LG Klinkenberg,NE Morrison,PJ Converse,E Nuermberger,J Grosset,DN McMurray,PC Karakousis,G Lamichhane,WR Bishai,

For similar bacterial infections and mycoses: bacterial infections: gram-positive bacterial infections: actinomycetales infections: mycobacterium infections: tuberculosis: tuberculosis, pulmonary research abstracts see: bacterial infections and mycoses: bacterial infections: gram-positive bacterial infections: actinomycetales infections: mycobacterium infections: tuberculosis: tuberculosis, pulmonary research

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Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of infectious diseases

VOLUME: 195

Page Numbers: 1634-42

Journal Abbreviation:

ISSN: 0022-1899

DAY: 23

MONTH: 04

YEAR: 2007

Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 413675

Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Keywords Mesh Terms:

KEYWORDS: Tuberculosis, Pulmonary

MESH TERMS: pathology

Chemical & Substance for Abstract: Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice. Information

Substance Name: DNA Transposable Elements

Registry Number: 0

Grant and Affiliation Information for Accelerated detection of Mycobacterium tuberculosis genes essential for bacterial survival in guinea pigs, compared with mice.

AFFILIATION: Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA. sjain5@jhmi.edu

Country: United States

AGENCY: United States NIAID

GRANT: N01 AI30036

ACRONYM: AI

MEDLINETA: J Infect Dis

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