Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis.

Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Research Abstract Details 

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Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Abstract Text:

K Hori,M Kanda,T Kurotsu,S Miura,Y Yamada,Y Saito,

The pantothenic acid content of gramicidin S synthetase 2(GS 2) was estimated microbiologically with enzymes obtained from the wild strain and gramicidin S-lacking mutant strains of Bacillus brevis. Four mutant enzymes from BI-4, C-3, E-1, and E-2 lacked pantothenic acid. Other mutant enzymes from BII-3, BI-3, BI-9, and BI-2 contained the same amount of pantothenic acid as the wild-type enzyme. Pantothenic acid-lacking GS 2 belonged to group V of mutant enzymes, which could activate all amino acids related to gramicidin S; their complementary enzyme, gramicidin S synthetase 1(GS 1), lacked racemizing activity. To ascertain whether 4'-phosphopantetheine is involved in the formation of D-phenylalanyl-L-prolyl diketopiperazine (DKP) and gramicidin S, combinations were tested of intact GS 1 from the wild strain with various mutant GS 2 either containing or lacking pantothenic acid. Only the combinations of wild-type GS 1 with mutant GS 2 containing pantothenic acid could synthesize DKP. Combinations with pantothenic acid-lacking GS 2 also failed to elongate peptide chains. Pantothenic acid-lacking GS 2 could bind the four amino acids which constitute gramicidin S as acyladenylates and thioesters, but the binding abilities were lower than those of the wild-type enzyme and other mutant enzymes containing the pantothenic group.

Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Publishing Authors By Initials

K Hori,M Kanda,T Kurotsu,S Miura,Y Yamada,Y Saito,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: piperazines research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: piperazines research

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Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 90

Page Numbers: 439-47

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1981

Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Keywords Mesh Terms:

KEYWORDS: Piperazines

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Absence of pantothenic acid in gramicidin S synthetase 2 obtained from some mutants of Bacillus brevis. Information

Substance Name: gramicidin-S-synthetase 2

Registry Number: EC 6.3.2.-

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Country: JAPAN

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MEDLINETA: J Biochem

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