Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii.

Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii. Research Abstract Details 

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Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii. Abstract Text:

Kisaburo Nagamune,Leslie M Hicks,Blima Fux,Fabien Brossier,Eduardo N Chini,L David Sibley,Kisaburo Nagamune,Leslie M Hicks,Blima Fux,Fabien Brossier,Eduardo N Chini,L David Sibley,

Calcium controls a number of critical events, including motility, secretion, cell invasion and egress by apicomplexan parasites. Compared to animal and plant cells, the molecular mechanisms that govern calcium signalling in parasites are poorly understood. Here we show that the production of the phytohormone abscisic acid (ABA) controls calcium signalling within the apicomplexan parasite Toxoplasma gondii, an opportunistic human pathogen. In plants, ABA controls a number of important events, including environmental stress responses, embryo development and seed dormancy. ABA induces production of the second-messenger cyclic ADP ribose (cADPR), which controls release of intracellular calcium stores in plants. cADPR also controls intracellular calcium release in the protozoan parasite T. gondii; however, previous studies have not revealed the molecular basis of this pathway. We found that addition of exogenous ABA induced formation of cADPR in T. gondii, stimulated calcium-dependent protein secretion, and induced parasite egress from the infected host cell in a density-dependent manner. Production of endogenous ABA within the parasite was confirmed by purification (using high-performance liquid chromatography) and analysis (by gas chromatography-mass spectrometry). Selective disruption of ABA synthesis by the inhibitor fluridone delayed egress and induced development of the slow-growing, dormant cyst stage of the parasite. Thus, ABA-mediated calcium signalling controls the decision between lytic and chronic stage growth, a developmental switch that is central in pathogenesis and transmission. The pathway for ABA production was probably acquired with an algal endosymbiont that was retained as a non-photosynthetic plastid known as the apicoplast. The plant-like nature of this pathway may be exploited therapeutically, as shown by the ability of a specific inhibitor of ABA synthesis to prevent toxoplasmosis in the mouse model.

Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii. Publishing Authors By Initials

K Nagamune,LM Hicks,B Fux,F Brossier,EN Chini,LD Sibley,K Nagamune,LM Hicks,B Fux,F Brossier,EN Chini,LD Sibley,

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Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature

VOLUME: 451

Page Numbers: 207-10

Journal Abbreviation: Nature

ISSN: 1476-4687

DAY: 10

MONTH: Jan

YEAR: 2008

Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Abscisic acid controls calcium-dependent egress and development in Toxoplasma gondii.

AFFILIATION: Department of Molecular Microbiology, Washington University School of Medicine, 660 S. Euclid Avenue, St Louis, Missouri 63110, USA.

Country: England

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MEDLINETA: Nature

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