Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts.

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Abstract Text:

Aaron J Bonk,Rongfeng Li,Liangxue Lai,Yanhong Hao,Zhonghua Liu,Melissa Samuel,Emily A Fergason,Kristin M Whitworth,Clifton N Murphy,Eric Antoniou,Randall S Prather,Aaron J Bonk,Rongfeng Li,Liangxue Lai,Yanhong Hao,Zhonghua Liu,Melissa Samuel,Emily A Fergason,Kristin M Whitworth,Clifton N Murphy,Eric Antoniou,Randall S Prather,

Early embryonic development in the pig requires DNA methylation remodeling of the maternal and paternal genomes. Aberrant remodeling, which can be exasperated by in vitro technologies, is detrimental to development and can result in physiological and anatomic abnormalities in the developing fetus and offspring. Here, we developed and validated a microarray based approach to characterize on a global scale the CpG methylation profiles of porcine gametes and blastocyst stage embryos. The relative methylation in the gamete and blastocyst samples showed that 18.5% (921/4,992) of the DNA clones were found to be significantly different (P < 0.01) in at least one of the samples. Furthermore, for the different blastocyst groups, the methylation profile of the in vitro-produced blastocysts was less similar to the in vivo-produced blastocysts as compared to the parthenogenetic- and somatic cell nuclear transfer (SCNT)-produced blastocysts. The microarray results were validated by using bisulfite sequencing for 12 of the genomic regions in liver, sperm, and in vivo-produced blastocysts. These results suggest that a generalized change in global methylation is not responsible for the low developmental potential of blastocysts produced by using in vitro techniques. Instead, the appropriate methylation of a relatively small number of genomic regions in the early embryo may enable early development to occur.

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Publishing Authors By Initials

AJ Bonk,R Li,L Lai,Y Hao,Z Liu,M Samuel,EA Fergason,KM Whitworth,CN Murphy,E Antoniou,RS Prather,AJ Bonk,R Li,L Lai,Y Hao,Z Liu,M Samuel,EA Fergason,KM Whitworth,CN Murphy,E Antoniou,RS Prather,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular reproduction and development

VOLUME: 75

Page Numbers: 250-64

Journal Abbreviation: Mol. Reprod. Dev.

ISSN: 1040-452X

DAY: 6

MONTH: Feb

YEAR: 2008

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8903333

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts.

AFFILIATION: Division of Animal Science, University of Missouri, Columbia, Missouri 65211, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Reprod Dev

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts Related Publications

• A protein tyrosine phosphatase inhibitor, sodium orthovanadate, causes parthenogenetic activation of pig oocytes via an increase in protein tyrosine kinase activity.
• Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced blastocysts.
• An anisotropy of human tactile sensitivity and its relation to the visual oblique effect.
• Carbohydrate tolerance and serum lipid responses to type of dietary carbohydrate and oral contraceptive use in young women.
• Cloned transgenic heart-healthy pork?
• Cloned transgenic swine via in vitro production and cryopreservation.
• Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos.
• Correlation of developmental differences of nuclear transfer embryos cells to the methylation profiles of nuclear transfer donor cells in Swine.
• Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion.
• Diacylglycerol-enhanced electrical activation of porcine oocytes matured in vitro.
• Electromagnetic modeling of active silicon nanocrystal waveguides.
• End note:summary.
• Fiber-to-waveguide coupler based on the parabolic reflector.
• High renal ectopy; a case report.
• Hyaluronic acid fillers for the male patient.
• Investigations of the diurnal cycle and mixing state of oxalic acid in individual particles in Asian aerosol outflow.
• Juvéderm: a hyaluronic acid dermal filler.
• Multichannel wavelength division multiplexing with photonic crystals.
• Nuclear remodeling and nuclear reprogramming for making transgenic pigs by nuclear transfer.
• Osteopontin improves in vitro development of porcine embryos and decreases apoptosis.
• Photoresist characterization and linearization procedure for the gray-scale fabrication of diffractive optical elements.
• Plasma fructose, uric acid, and inorganic phosphorus responses of hyperinsulinemic men fed fructose.
• Pluristem Therapeutics, Inc.
• Pneumonephrosis; a complication of necrotizing pyelonephritis.
• Real-time, single-particle measurements of oligomers in aged ambient aerosol particles.
• Surgical treatment of serous cyst of the kidney.
• Targeted genetic modification: xenotransplantation and beyond.
• The CB2 cannabinoid agonist AM-1241 prolongs survival in a transgenic mouse model of amyotrophic lateral sclerosis when initiated at symptom onset.
• Thin film solar cell design based on photonic crystal and diffractive grating structures.
• Vector-based plane-wave spectrum method for the propagation of cylindrical electromagnetic fields.