AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice.

AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice. Research Abstract Details 

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AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice. Abstract Text:

Takashi Kawakami,Hideaki Kanazawa,Toru Satoh,Masaki Ieda,Yasuyo Ieda,Kensuke Kimura,Hideki Mochizuki,Takashi Shimada,Chieko Yokoyama,Satoshi Ogawa,Tadashi Tanabe,Keiichi Fukuda,Takashi Kawakami,Hideaki Kanazawa,Toru Satoh,Masaki Ieda,Yasuyo Ieda,Kensuke Kimura,Hideki Mochizuki,Takashi Shimada,Chieko Yokoyama,Satoshi Ogawa,Tadashi Tanabe,Keiichi Fukuda,Takashi Kawakami,Hideaki Kanazawa,Toru Satoh,Masaki Ieda,Yasuyo Ieda,Kensuke Kimura,Hideki Mochizuki,Takashi Shimada,Chieko Yokoyama,Satoshi Ogawa,Tadashi Tanabe,Keiichi Fukuda,

Although prostaglandin I2 is used to treat pulmonary hypertension (PH), continuous intravenous administration is necessary. We investigated whether human PGIS (hPGIS) gene transfer using adeno-associated virus (AAV) vector was effective in treating an animal model of PH. PH was induced by subjecting mice to 10% O(2). Type 1-AAV-hPGIS was injected into the left thigh muscle after 24h. Significant PH was induced at 8 weeks, but AAV-hPGIS administration significantly inhibited the increase in RV systolic pressure. PH-induced BNP up-regulation in the RV was reduced to the control level. The severe medial thickening of pulmonary arteries in PH was significantly suppressed by AAV-hPGIS. The hPGIS gene was detected only on the injected side. No pathological changes were observed at the injected site. At 24 weeks, all PH mice were deceased, but 47% of AAV-hPGIS-treated mice survived. This study demonstrated that AAV-hPGIS administration was effective in treating PH and prolonging survival.

AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice. Publishing Authors By Initials

T Kawakami,H Kanazawa,T Satoh,M Ieda,Y Ieda,K Kimura,H Mochizuki,T Shimada,C Yokoyama,S Ogawa,T Tanabe,K Fukuda,T Kawakami,H Kanazawa,T Satoh,M Ieda,Y Ieda,K Kimura,H Mochizuki,T Shimada,C Yokoyama,S Ogawa,T Tanabe,K Fukuda,T Kawakami,H Kanazawa,T Satoh,M Ieda,Y Ieda,K Kimura,H Mochizuki,T Shimada,C Yokoyama,S Ogawa,T Tanabe,K Fukuda,

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AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemical and biophysical research communication

VOLUME: 363

Page Numbers: 656-61

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 20

MONTH: 09

YEAR: 2007

AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice.

AFFILIATION: Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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