A Whole Body, In Vivo, Fatty Acid Balance Method to Quantify PUFA Metabolism (Desaturation, Elongation and Beta-oxidation).

A Whole Body, In Vivo, Fatty Acid Balance Method to Quantify PUFA Metabolism (Desaturation, Elongation and Beta-oxidation). Research Abstract Details 

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A Whole Body, In Vivo, Fatty Acid Balance Method to Quantify PUFA Metabolism (Desaturation, Elongation and Beta-oxidation). Abstract Text:

Giovanni M Turchini,David S Francis,Sena S De Silva,Giovanni M Turchini,David S Francis,Sena S De Silva,Giovanni M Turchini,David S Francis,Sena S De Silva,

Currently there are several contrasting methods utilized for estimating elongation and desaturation of fatty acids and their general metabolism. The majority of these methods involve an ex vivo approach, requiring expensive and sophisticated equipment, likely to result in considerable variation in enzyme activity between and within species. In the present paper we introduce a further development of the whole-body fatty acid balance method for the estimation of the elongation and desaturation of fatty acids. This method though receiving considerable attention because of its simplicity and reliability has yet to be presented in detail. Theoretically, the whole-body fatty acid balance method can potentially be applied to any organism and requires little more than a gas chromatography unit for fatty acid analysis and elementary calculations. As such in this paper we attempt to spell out in detail the theoretical basis and the methods of application drawing specific examples. Using the present method it is possible to measure the fate of individual fatty acids towards desaturation, elongation and oxidation and calculate the elongase, Delta-6 desaturase and Delta-5 desaturase activities.

A Whole Body, In Vivo, Fatty Acid Balance Method to Quantify PUFA Metabolism (Desaturation, Elongation and Beta-oxidation). Publishing Authors By Initials

GM Turchini,DS Francis,SS De Silva,GM Turchini,DS Francis,SS De Silva,GM Turchini,DS Francis,SS De Silva,

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A Whole Body, In Vivo, Fatty Acid Balance Method to Quantify PUFA Metabolism (Desaturation, Elongation and Beta-oxidation). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Lipids

VOLUME: 42

Page Numbers: 1065-71

Journal Abbreviation: Lipids

ISSN: 0024-4201

DAY: 16

MONTH: 08

YEAR: 2007

A Whole Body, In Vivo, Fatty Acid Balance Method to Quantify PUFA Metabolism (Desaturation, Elongation and Beta-oxidation). Information

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LANGUAGE: eng

NlmUniqueID: 60450

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AFFILIATION: School of Life & Environmental Sciences, Deakin University, PO Box 423, Warrnambool, VIC, 3280, Australia, giovanni.turchini@deakin.edu.au.

Country: United States

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MEDLINETA: Lipids

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