A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities.

A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Research Abstract Details 

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A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Abstract Text:

Kirk Beebe,William Waas,Zhanna Druzina,Min Guo,Paul Schimmel,

Aminoacyl transfer RNA (tRNA) synthetases are intensely studied enzymes because of their importance in the establishment of the genetic code and their connection to disease and medicine. During the advancement of this field, several assays were developed. Despite many innovations, the sensitivity, simplicity, and reliability of the radiometric assays (which were among the first to be developed) have ensured their continued use. Four activities are measured by these assays: active site titration, amino acid activation, aminoacylation, and posttransfer editing (deacylation). In an effort to maintain the advantage of these assays while enhancing throughput, reducing waste, and improving data quality, a universal 96-well filter plate format was developed. This format facilitates the assays for all four of the widely studied activities.

A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Publishing Authors By Initials

K Beebe,W Waas,Z Druzina,M Guo,P Schimmel,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Analytical biochemistry

VOLUME: 368

Page Numbers: 111-21

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 18

MONTH: 05

YEAR: 2007

A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Information

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LANGUAGE: eng

NlmUniqueID: 370535

A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: methods

Chemical & Substance for Abstract: A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities. Information

Substance Name: Amino Acyl-tRNA Synthetases

Registry Number: EC 6.1.1.-

Grant and Affiliation Information for A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities.

AFFILIATION: Department of Molecular Biology and Chemistry and Skaggs Institute for Chemical Biology, Scripps Research Institute, La Jolla, CA 92037, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 15539

ACRONYM: GM

MEDLINETA: Anal Biochem

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