A unique DNA binding domain converts T-cell factors into strong Wnt effectors.

A unique DNA binding domain converts T-cell factors into strong Wnt effectors. Research Abstract Details 

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A unique DNA binding domain converts T-cell factors into strong Wnt effectors. Abstract Text:

Fawzia A Atcha,Adeela Syed,Beibei Wu,Nate P Hoverter,Noriko N Yokoyama,Ju-Hui T Ting,Jesus E Munguia,Harry J Mangalam,J Lawrence Marsh,Marian L Waterman,Fawzia A Atcha,Adeela Syed,Beibei Wu,Nate P Hoverter,Noriko N Yokoyama,Ju-Hui T Ting,Jesus E Munguia,Harry J Mangalam,J Lawrence Marsh,Marian L Waterman,

Wnt regulation of gene expression requires binding of LEF/T-cell factor (LEF/TCF) transcription factors to Wnt response elements (WREs) and recruitment of the activator beta-catenin. There are significant differences in the abilities of LEF/TCF family members to regulate Wnt target genes. For example, alternatively spliced isoforms of TCF-1 and TCF-4 with a C-terminal "E" tail are uniquely potent in their activation of LEF1 and CDX1. Here we report that the mechanism responsible for this unique activity is an auxiliary 30-amino-acid DNA interaction motif referred to here as the "cysteine clamp" (or C-clamp). The C-clamp contains invariant cysteine, aromatic, and basic residues, and surface plasmon resonance (SPR) studies with recombinant C-clamp protein showed that it binds double-stranded DNA but not single-stranded DNA or RNA (equilibrium dissociation constant = 16 nM). CASTing (Cyclic Amplification and Selection of Targets) experiments were used to test whether this motif influences WRE recognition. Full-length LEF-1, TCF-1E, and TCF-1E with a mutated C-clamp all bind nearly identical WREs (TYYCTTTGATSTT), showing that the C-clamp does not alter WRE specificity. However, a GC element downstream of the WRE (RCCG) is enriched in wild-type TCF-1E binding sites but not in mutant TCF-1E binding sites. We conclude that the C-clamp is a sequence-specific DNA binding motif. C-clamp mutations destroy the ability of beta-catenin to regulate the LEF1 promoter, and they severely impair the ability of TCF-1 to regulate growth in colon cancer cells. Thus, E-tail isoforms of TCFs utilize two DNA binding activities to access a subset of Wnt targets important for cell growth.

A unique DNA binding domain converts T-cell factors into strong Wnt effectors. Publishing Authors By Initials

FA Atcha,A Syed,B Wu,NP Hoverter,NN Yokoyama,JH Ting,JE Munguia,HJ Mangalam,JL Marsh,ML Waterman,FA Atcha,A Syed,B Wu,NP Hoverter,NN Yokoyama,JH Ting,JE Munguia,HJ Mangalam,JL Marsh,ML Waterman,

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A unique DNA binding domain converts T-cell factors into strong Wnt effectors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 8352-63

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 1098-5549

DAY: 24

MONTH: 09

YEAR: 2007

A unique DNA binding domain converts T-cell factors into strong Wnt effectors. Information

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LANGUAGE: eng

NlmUniqueID: 8109087

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Grant and Affiliation Information for A unique DNA binding domain converts T-cell factors into strong Wnt effectors.

AFFILIATION: Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD36081

ACRONYM: HD

MEDLINETA: Mol Cell Biol

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