A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes.

A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Research Abstract Details 

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A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Abstract Text:

Zhongxia Li,Xinyan Zhao,Chenghui Zhou,Baiyan Gu,Fred R Frankel,

Listeria monocytogenes (Lm) is a Gram-positive intracellular pathogen that can elicit strong cellular immunity. An attenuated strain (Lmdd) with deletions in two genes (dal and dat) required for d-alanine synthesis and viability has been shown to induce long-lived protective systemic and mucosal immune responses in mice when administered in the presence of the required amino acid. To bypass the necessity for exogenous d-alanine without compromising the safety of the original strain, the defect of Lmdd was complemented with a heterologous Bacillus subtilis dal gene, and the effects of truncating the upstream region of the gene on its transcription efficiency and of modifying its protein product with an ssrA tag at the 3'-terminus were examined. The strains with 551 bp and 80 bp upstream regions showed high levels of transcription and grew without d-alanine. The strains with the shortest upstream regions, 48 bp and 18 bp, showed greatly decreased levels of transcription and failed to grow in the absence of d-alanine. Addition of an ssrA tag to the longer genes resulted in a somewhat altered growth pattern in media and a reduced plaque size on L2 fibroblasts. These bacteria contained low levels of racemase protein and reduced free pools of d-alanine. One of the strains tested further, Lmdd/pA80S, was rapidly cleared from the spleens of infected mice but nevertheless induced a strong immune response that protected mice against challenge by wild-type L. monocytogenes. These bacteria can thus induce immune responses in mice comparable to the original Lmdd strain, but without the need for exogenous d-alanine, and may have use as a live vaccine vector against infectious diseases and cancers.

A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Publishing Authors By Initials

Z Li,X Zhao,C Zhou,B Gu,FR Frankel,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

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A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Microbiology (Reading, England)

VOLUME: 152

Page Numbers: 3091-102

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 3

MONTH: Oct

YEAR: 2006

A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: genetics

Chemical & Substance for Abstract: A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes. Information

Substance Name: Alanine Racemase

Registry Number: EC 5.1.1.1

Grant and Affiliation Information for A truncated Bacillus subtilis dal gene with a 3' ssrA gene tag regulates the growth and virulence of racemase-deficient Listeria monocytogenes.

AFFILIATION: Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI 42509

ACRONYM: AI

MEDLINETA: Microbiology

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