A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation.

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Abstract Text:

Miriam M Kleiter,Daohai Yu,Lenore A Mohammadian,Nelsen Niehaus,Ivan Spasojevic,Linda Sanders,Benjamin L Viglianti,Pavel S Yarmolenko,Marlene Hauck,Neil A Petry,Terence Z Wong,Mark W Dewhirst,Donald E Thrall,

PURPOSE: A noninvasive method to monitor intratumoral Doxil delivery in individual patients during targeted tumor therapy is important to predict treatment response. The purpose of this study was to determine if a small tracer dose of technetium-99m (99mTc)-labeled liposomes could be used to quantify the effect of local hyperthermia on intratumoral Doxil extravasation. EXPERIMENTAL DESIGN: Experiments were carried out in a rat fibrosarcoma model with transplanted thigh tumors. Liposomes of approximately same size and composition as Doxil were radiolabeled using [technetium-99m (99mTc)]exametazime. Eight treatment groups received either Doxil, a tracer dose or a large dose of 99mTc-labeled liposomes, or a combination of tracer and Doxil, with or without hyperthermia. This design was chosen to assure that coadministration of both liposomal formulations did not influence their intratumoral distribution. Hyperthermia was done for 45 minutes. Scintigraphic images were obtained at 5 and 18 hours. At 18 hours, tumors were removed and gamma counts as well as doxorubicin concentrations were measured. RESULTS: Intratumoral extravasation of the 99mTc-labeled tracer could be imaged scintigraphically under normothermic and hyperthermic conditions. The thermal enhancement ratio was slightly higher for radiolabeled liposomes than for doxorubicin concentration. However, there was a significant positive correlation of intratumoral doxorubicin concentration and intratumoral uptake of the radiolabeled tracer (expressed as percentage of the injected dose per gram of tissue). Coadministration of radiolabeled liposomes did not negatively influence the amount of drug delivered with Doxil. CONCLUSIONS: The use of a radiolabeled tracer has potential value to monitor drug delivery and estimate the effect of an intervention aimed to increase liposomal accumulation, such as local hyperthermia.

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Publishing Authors By Initials

MM Kleiter,D Yu,LA Mohammadian,N Niehaus,I Spasojevic,L Sanders,BL Viglianti,PS Yarmolenko,M Hauck,NA Petry,TZ Wong,MW Dewhirst,DE Thrall,

For similar xenograft model antitumor assays research abstracts see: xenograft model antitumor assays research

PUBMED ID PMID:

MEDLINE DATE:

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Clinical cancer research : an official journal of

VOLUME: 12

Page Numbers: 6800-7

Journal Abbreviation: Clin. Cancer Res.

ISSN: 1078-0432

DAY: 15

MONTH: Nov

YEAR: 2006

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9502500

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Keywords Mesh Terms:

KEYWORDS: Xenograft Model Antitumor Assays

MESH TERMS: radiation effects

Chemical & Substance for Abstract: A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation. Information

Substance Name: Doxorubicin

Registry Number: 23214-92-8

Grant and Affiliation Information for A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation.

AFFILIATION: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA42745

ACRONYM: CA

MEDLINETA: Clin Cancer Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation Related Publications

• "An open invitation to empirical biologists".
• A tracer dose of technetium-99m-labeled liposomes can estimate the effect of hyperthermia on intratumoral doxil extravasation.
• Accumulation of regulatory T cells in local draining lymph nodes of the lung correlates with spontaneous resolution of chronic asthma in a murine model.
• CT usage.
• Capital planning in radiology.
• Changing Relationships between Radiologists and Hospitals * Part II. Contracts and Resolution of Issues.
• Changing relationships between radiologists and hospitals. Part I. Background and major issues.
• Commentary on "are we really practicing medicine today?".
• Data merging for integrated microarray and proteomic analysis.
• Design & construction. The quake is fake, but the lessons are real.
• Directions in radiology for the next millennium.
• Effect of advances in CT on practice.
• Effect of multislice CT technology on scanner productivity.
• Emergency department. Paramedics take a more active role in treating heart attacks.
• Factors warranting increased spending for CT.
• Making global telemedicine practical and affordable: demonstrations from the Middle East.
• Management. Employee satisfaction can improve despite rise in premiums and CO-pays.
• Murine cytomegalovirus influences foxj1 expression, ciliogenesis, and mucus plugging in mice with allergic airway disease.
• Nursing. Residency program helps rural hospitals attract, keep nurses.
• Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.
• Physician recruitment. Garrison Keillor: doctor magnet.
• Physicians. Country doctor saves small-town hospital.
• Procedures that may be replaced by CT.
• Progress and complexity for the next century.
• Prospects for patient procedural volume.
• Quality. And the Baldrige goes to: Mercy and Sharp.
• Referring physicians' awareness of CT benefits.
• Rural recruitment.
• Staffing. Nurse unionizing effort fails as hospital leaders listen--and respond--to staff woes.
• The use of PACS.