A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling.

A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Research Abstract Details 

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A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Abstract Text:

David Simpson,Hong Liu,Tai-Hwang Michael Fan,Robert Nerem,Samuel C Dudley,

Cell replacement therapy has become an attractive solution for myocardial repair. Typical cell delivery techniques, however, suffer from poor cell engraftment and inhomogeneous cell distributions. Therefore, we assessed the hypothesis that an epicardially applied, tissue-engineered cardiac patch containing progenitor cells would result in enhanced exogenous cell engraftment. Human mesenchymal stem cells (hMSCs) were embedded into a rat tail type I collagen matrix to form the cardiac patch. Myocardial infarction was induced by left anterior descending coronary artery ligation in immunocompetent male cesarean-derived fischer rats, and patches with or without cells were secured to hearts with fibrin sealant. After patch formation, hMSCs retained a viability of >90% over 5 days in culture. In addition, >75% of hMSCs maintained a high degree of potency prior to patch implantation. After 4 days in culture, patches were applied to the epicardial surface of the infarct area and resulted in 23% +/- 4% engraftment of hMSCs at 1 week (n = 6). Patch application resulted in a reduction in left ventricle interior diameter at systole, increased anterior wall thickness, and a 30% increase in fractional shortening. Despite this improvement in myocardial remodeling, hMSCs were not detectable at 4 weeks after patch application, implying that improvement did not require long-term cell engraftment. Patches devoid of progenitor cells showed no improvement in remodeling. In conclusion, pluripotent hMSCs can be efficiently delivered to a site of myocardial injury using an epicardial cardiac patch, and such delivery results in improved myocardial remodeling after infarction. Disclosure of potential conflicts of interest is found at the end of this article.

A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Publishing Authors By Initials

D Simpson,H Liu,TH Fan,R Nerem,SC Dudley,

For similar circulatory and respiratory physiology: cardiovascular physiology: cardiovascular physiologic processes: ventricular function: ventricular remodeling research abstracts see: circulatory and respiratory physiology: cardiovascular physiology: cardiovascular physiologic processes: ventricular function: ventricular remodeling research

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A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 2350-7

Journal Abbreviation: Stem Cells

ISSN: 1549-4918

DAY: 24

MONTH: 05

YEAR: 2007

A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9304532

A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Keywords Mesh Terms:

KEYWORDS: Ventricular Remodeling

MESH TERMS: methods

Chemical & Substance for Abstract: A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling. Information

Substance Name: smooth muscle actin, rat

Registry Number: 0

Grant and Affiliation Information for A tissue engineering approach to progenitor cell delivery results in significant cell engraftment and improved myocardial remodeling.

AFFILIATION: Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL73753

ACRONYM: HL

MEDLINETA: Stem Cells

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