A thermostable Gm-methylase recognizes the tertiary structure of tRNA.

A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Research Abstract Details 

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A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Abstract Text:

T Matsumoto,T Ohta,I Kumagai,T Oshima,K Murao,T Hasegawa,H Ishikura,K Watanabe,

The efficiency of methylation of tRNA by a thermostable tRNA(guanosine-2')-methyltransferase (Gm-methylase) was examined at various temperatures using several species of tRNA isolated from Escherichia coli, yeast and Bacillus subtilis, each possessing different thermal properties. The optimal temperature for the methylation reaction was ca. 20 degrees C lower than the melting temperature of the tRNA in each case. Arrhenius plots of the methylation reactions with various tRNAs gave straight lines below the optimal temperatures in all cases, with similar activation energies of between 10 and 14 kcal/mol. Above the optimal temperatures, the methyl acceptor activity decreased as the incubation temperature was raised to 80 degrees C, at which point the methylase was still active. A correlation was observed between the remaining methyl acceptor activity and the hyperchromicity of tRNA. These results suggest that Gm-methylase recognizes the tertiary structure of tRNA, and it is not the substrate tRNA but the enzyme which is activated by heat.

A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Publishing Authors By Initials

T Matsumoto,T Ohta,I Kumagai,T Oshima,K Murao,T Hasegawa,H Ishikura,K Watanabe,

For similar enzymes and coenzymes: enzymes: transferases: one-carbon group transferases: methyltransferases: trna methyltransferases research abstracts see: enzymes and coenzymes: enzymes: transferases: one-carbon group transferases: methyltransferases: trna methyltransferases research

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A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 101

Page Numbers: 1191-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1987

A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Keywords Mesh Terms:

KEYWORDS: tRNA Methyltransferases

MESH TERMS: metabolism

Chemical & Substance for Abstract: A thermostable Gm-methylase recognizes the tertiary structure of tRNA. Information

Substance Name: tRNA (guanosine-2'-O-)-methyltransferase

Registry Number: EC 2.1.1.34

Grant and Affiliation Information for A thermostable Gm-methylase recognizes the tertiary structure of tRNA.

AFFILIATION: Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

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