A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals.

A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Research Abstract Details 

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A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Abstract Text:

S Miyagawa,M Yamada,K Matsunami,Y Koresawa,M Ikawa,M Okabe,R Shirakura,

The human DAF (CD55) gene was chosen as a representative molecule in a xenotransplantation study. The gene was synthesized in order to adapt its codons to those which are more frequent in mammals, especially pigs, and the expression levels were then examined in Chinese hamster ovarian (CHO) cells, swine endothelial cell (SEC) and transgenic mice. A significant increase in protein production with no detectable mRNA elevation was observed in the transfectants of synthetic DAF (sDAF), compared with the wild-type DAF (wtDAF) and delta-SCR1 wild-type DAF (Delta1wtDAF). Consistent with the in vitro data, the expression of DAF in mice that carry sDAF was higher than Delta1wtDAF in many organs, especially the pancreas. The sDAF showed a high level of expression in SEC and transgenic mice, suggesting that it will be useful in the development of transgenic pigs with high levels of expression.

A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Publishing Authors By Initials

S Miyagawa,M Yamada,K Matsunami,Y Koresawa,M Ikawa,M Okabe,R Shirakura,

For similar surgical procedures, operative: transplantation: transplantation, heterologous research abstracts see: surgical procedures, operative: transplantation: transplantation, heterologous research

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A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 129

Page Numbers: 795-801

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2001

A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Keywords Mesh Terms:

KEYWORDS: Transplantation, Heterologous

MESH TERMS: methods

Chemical & Substance for Abstract: A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. Information

Substance Name: Codon

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Grant and Affiliation Information for A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals.

AFFILIATION: Division of Organ Transplantation, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka 565-0871, Japan. miyagawa@orgtrp.med.osaka-u.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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