A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells.

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Abstract Text:

Jing Ge,Yishu Huang,Xiaotong Hu,Jiang Zhong,

A short peptide motif from gp350/220 of Epstein-Barr virus, EDPGFFNVEI, which was known to bind to CD21, a surface protein on B-lymphocyte, was inserted into the baculovirus surface protein gp64. The recombinant virus carrying the hybrid gp64/gp350 gene, vAc-gp350EGFP, was obtained, and the expression of gp64/gp350 protein was confirmed with immunoblot using anti-gp350 antibody. When compared with a control virus with wild type gp64, vAc-gp350EGFP showed increased transduction efficiency in B cell lines Raji, HR1, B95-8, BJAB, and DG75, regardless of their being EBV-positive or EBV-negative. No such increase was seen in non-B cell lines HEK293 and HeLa. When Raji cells were transduced with increased amount of vAc-gp350EGFP, transduction became saturated when the multiplicity of infection was higher than 20pfu/cell. The transduction of Raji cells by vAc-gp350EGFP was dose-dependently inhibited by pre-treatment of cells with anti-CD21 antibody. These results showed that vAc-gp350EGFP entered B cells by interacting with CD21.

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Publishing Authors By Initials

J Ge,Y Huang,X Hu,J Zhong,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

PUBMED ID PMID:

MEDLINE DATE:

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biotechnology

VOLUME: 129

Page Numbers: 367-72

Journal Abbreviation:

ISSN: 0168-1656

DAY: 24

MONTH: 02

YEAR: 2007

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8411927

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells.

AFFILIATION: Department Of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A surface-modified baculovirus vector with improved gene delivery to B-lymphocytic cells Related Publications

• Expression and purification of recombinant bone morphogenetic protein-2 in E.coli.
• [A clinical analysis of 30 cases of sympathetic ophthalmia]
• [An epidemiologic study of cough in young college students in Guangzhou]
• [CD4+CD25+ T lymphocytes in peripheral blood from patients with asthma]
• [Clinical observation on liang's anti-rheumatism and rheumatoid granule in rheumatoid arthritis in the active stage]
• [Deep fungal infections: new challenges and prospective]
• [Detection and related analysis to chromosome 22q11 microdeletion in patients with congenital heart diseases]
• [Determination of atomization efficiency in graphite furnace with V-boat]
• [Determination of metronidazole in gastric juice by high performance liquid chromatography]
• [Disparity of asthma prevalence in Chinese schoolchildren is due to differences in lifestyle factors]
• [Etiology and a diagnostic protocol for patients with chronic cough]
• [Expiratory flow limitation detected by negative expiratory pressure in patients with chronic obstructive pulmonary disease]
• [Expressions of L-type calcium channel and potassium channel Kv4.3 in rapid paced primary cultured atrial myocytes]
• [Finite element analysis of lumbar facet joint contact model]
• [Fluorescence spectroscopic analysis of huminite of the peat and soft brown coal]
• [Influence of extraction on fluorescence of the subrenite in the peat]
• [Inhibitory effect of grape procyanidin on the cell apoptosis induced by radiation]
• [Investigation of proximal femoral marrow with magnetic resonance imaging in recovered patients with severe acute respiratory syndrome]
• [Observation of the effect of hyperoxic sodium chloride solution on hypoxic injury]
• [Progress in the studies on hydrogel burn dressings]
• [Protective effect of specific antibody in serum of convalescent patient with SARS]
• [Research on quantified diagnosis and combining diseases with syndrome of blood stasis]
• [Studies on pathogenesis of primary biliary cirrhosis]
• [Subcutaneous implantation of tissue-engineered heart valve leaflets in nude mice]
• [The advance of research for biocompatibility of medical polyurethanes]
• [The epidemiology and management of influenza]
• [The protective effect of Rhadiola Astragalus Codonopsis Compound on myocardium in early stage of severe burn at plateau in rats]
• [The relationship between mouth pressure or tracheal pressure and esophagus pressure and diaphragmatic pressure]
• [Time trends in the prevalence of asthma and allergic diseases over 7 years among adolescents in Guangzhou city]
• [Uprighting of mesially or horizontally impacted mandibular molars]