A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry.

A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Research Abstract Details 

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A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Abstract Text:

Ruimin Xie,Willie Johnson,Lorna Rodriguez,Murugesan Gounder,Gene S Hall,Brian Buckley,

To study the carboplatin-protein interaction, a sensitive method using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS) was developed. The complexes formed between plasma proteins and carboplatin were monitored and identified with this method. Composite blood plasma samples from patients who were undergoing chemotherapy were analyzed, and carboplatin was found to bind plasma proteins. In addition, blank plasma samples were spiked with carboplatin and were analyzed as a time course study, and the results confirmed that carboplatin formed complexes with plasma proteins, primarily albumin and gamma-globulin. To further substantiate the study, these two proteins were incubated with carboplatin. The binding between carboplatin and these proteins was then characterized qualitatively and quantitatively. In addition to a one-to-one binding of Pt to protein, protein aggregation was observed. The kinetics of the binding process of carboplatin to albumin and gamma-globulin was also studied. The initial reaction rate constant of carboplatin binding to albumin was determined to be 0.74 M(-1) min(-1), while that for gamma-globulin was 1.01 M(-1) min(-1), which are both lower than the rate constant of the cisplatin-albumin reaction previously reported.

A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Publishing Authors By Initials

R Xie,W Johnson,L Rodriguez,M Gounder,GS Hall,B Buckley,

For similar natural sciences: weights and measures: reference standards research abstracts see: natural sciences: weights and measures: reference standards research

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A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical and bioanalytical chemistry

VOLUME: 387

Page Numbers: 2815-22

Journal Abbreviation:

ISSN: 1618-2642

DAY: 6

MONTH: 03

YEAR: 2007

A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Information

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LANGUAGE: eng

NlmUniqueID: 101134327

A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Reference Standards

MESH TERMS: methods

Chemical & Substance for Abstract: A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry. Information

Substance Name: Carboplatin

Registry Number: 41575-94-4

Grant and Affiliation Information for A study of the interactions between carboplatin and blood plasma proteins using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry.

AFFILIATION: Environmental and Occupational Health Sciences Institute, Rutgers, The State University of New Jersey, 170 Frelinghuysen Road, Piscataway, NJ 08854, USA.

Country: Germany

AGENCY: United States NIEHS

GRANT: ES05022

ACRONYM: ES

MEDLINETA: Anal Bioanal Chem

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