A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA.

A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Research Abstract Details 

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A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Abstract Text:

Manjori Ganguly,Feng Wang,Mahima Kaushik,Michael P Stone,Luis A Marky,Barry Gold,

The incorporation of 7-deazaguanine modifications into DNA is frequently used to probe protein recognition of H-bonding information in the major groove of DNA. While it is generally assumed that 7-deazaguanine forms a normal Watson-Crick base pair with cytosine, detailed thermodynamic and structural analyses of this modification have not been reported. The replacement of the 7-N atom on guanine with a C-H, alters the electronic properties of the heterocycle and eliminates a major groove cation-binding site that could affect the organization of salts and water in the major groove. We report herein the characterization of synthetic DNA oligomers containing 7-deazaguanine using a variety of complementary approaches: UV thermal melting, differential scanning calorimetry (DSC), circular dichroism (CD), chemical probing and NMR. The results indicate that the incorporation of a 7-deazaguanine modification has a significant effect on the dynamic structure of the DNA at the flanking residue. This appears to be mediated by changes in hydration and cation organization.

A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Publishing Authors By Initials

M Ganguly,F Wang,M Kaushik,MP Stone,LA Marky,B Gold,

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A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 6181-95

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 12

MONTH: 09

YEAR: 2007

A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Information

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LANGUAGE: eng

NlmUniqueID: 411011

A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Keywords Mesh Terms:

KEYWORDS: Water

MESH TERMS: chemistry

Chemical & Substance for Abstract: A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA. Information

Substance Name: Deoxyguanosine

Registry Number: 961-07-9

Grant and Affiliation Information for A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA.

AFFILIATION: Eppley Institute for Research in Cancer, Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE-68198-6025, USA.

Country: England

AGENCY: United States NCI

GRANT: P20 CA36727

ACRONYM: CA

MEDLINETA: Nucleic Acids Res

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