A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal.

A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Research Abstract Details 

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A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Abstract Text:

Ute ,Brian J Hare,Breck A Duerkop,Martin Schuster,Brian L Hanzelka,Roger Heim,Eric R Olson,E Peter Greenberg,

The pathogenic bacterium Pseudomonas aeruginosa uses acyl-homoserine lactone quorum-sensing signals to coordinate the expression of a battery of virulence genes in a cascade of regulatory events. The quorum-sensing signal that triggers the cascade is N-3-oxo-dodecanoyl homoserine lactone (3OC12-HSL), which interacts with two signal receptor-transcription factors, LasR and QscR. This signal is base labile, and it is degraded by mammalian PON lactonases. We have identified a structurally unrelated triphenyl mimic of 3OC12-HSL that is base-insensitive and PON-resistant. The triphenyl mimic seems to interact specifically with LasR but not with QscR. In silico analysis suggests that the mimic fits into the 3OC12-HSL-binding site of LasR and makes key contacts with LasR. The triphenyl mimic is an excellent scaffold for developing quorum-sensing inhibitors, and its stability and potency make it ideal for biotechnology uses such as heterologous gene expression.

A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Publishing Authors By Initials

U ,BJ Hare,BA Duerkop,M Schuster,BL Hanzelka,R Heim,ER Olson,EP Greenberg,

For similar proteins: dna-binding proteins: trans-activators research abstracts see: proteins: dna-binding proteins: trans-activators research

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A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 16948-52

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 30

MONTH: 10

YEAR: 2006

A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Keywords Mesh Terms:

KEYWORDS: Trans-Activators

MESH TERMS: metabolism

Chemical & Substance for Abstract: A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal. Information

Substance Name: 4-Butyrolactone

Registry Number: 96-48-0

Grant and Affiliation Information for A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal.

AFFILIATION: Vertex Pharmaceuticals Incorporated, 130 Waverly Street, Cambridge, MA 02139, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32 GM 07270

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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