A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6.

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Abstract Text:

Zeljka Smit-McBride,Sharon L Oltjen,Matthew M Lavail,Leonard M Hjelmeland,

PURPOSE: Hyperoxia-related retinal degeneration (HRRD) is a model system in the mouse in which elevated oxygen levels are used to induce retinal degeneration. The hypothesis for the present study was that strain differences in HRRD susceptibility are due to allelic variants of one or more genes in the mouse genome whose human orthologues should be important targets for research and drug development. METHODS: C57BL/6J, A/J, or B.A-Chr6 mice were exposed to 75% oxygen (hyperoxia) or room air for 14 days. After death, one eye was fixed and processed for outer nuclear layer (ONL) thickness measurements. The retina and RPE/choroid were separately dissected from the fellow eye and processed for microarray analysis. Single nucleotide polymorphism (SNP) analysis for transcribed sequences from the C57BL/6J and A/J genomes was conducted using the NIH genome site. RESULTS: C57BL/6J mice developed a significant retinal degeneration in the inferior hemisphere after 14 days of hyperoxia. Under identical conditions, A/J mice exhibited only minor changes. A significant genetic effect was located on chromosome 6. SNP analysis of known transcribed sequences on chromosome 6 combined with microarray expression analysis yielded 33 candidate genes. CONCLUSIONS: A significant genetic effect of susceptibility to HRRD is located on chromosome 6. In silico analysis of transcribed sequences results in a fairly small number of candidate genes.

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Publishing Authors By Initials

Z Smit-McBride,SL Oltjen,MM Lavail,LM Hjelmeland,

For similar eye diseases: retinal diseases: retinal degeneration research abstracts see: eye diseases: retinal diseases: retinal degeneration research

PUBMED ID PMID:

MEDLINE DATE:

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Investigative ophthalmology & visual science

VOLUME: 48

Page Numbers: 405-11

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 3

MONTH: Jan

YEAR: 2007

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Keywords Mesh Terms:

KEYWORDS: Retinal Degeneration

MESH TERMS: pathology

Chemical & Substance for Abstract: A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6. Information

Substance Name: Oxygen

Registry Number: 7782-44-7

Grant and Affiliation Information for A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6.

AFFILIATION: Department of Ophthalmology, Vitreoretinal Research Lab, University of California at Davis, School of Medicine, Davis, California 95616, USA.

Country: United States

AGENCY: United States NEI

GRANT: R01EY01919

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6 Related Publications

• A strong genetic determinant of hyperoxia-related retinal degeneration on mouse chromosome 6.