A strategy to develop fast RP-HPLC methods using monolithic silica columns.

A strategy to develop fast RP-HPLC methods using monolithic silica columns. Research Abstract Details 

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A strategy to develop fast RP-HPLC methods using monolithic silica columns. Abstract Text:

Sami El Deeb,Lutz Preu,Hermann ,

Since the appearance of monolithic silica, much work has been done describing the properties of monolithic silica columns. Meanwhile the transferability of analytical methods from conventional to monolithic silica columns has been intensively investigated [1-5]. RP HPLC method development strategies for conventional columns should be updated or scaled to meet the higher performing monolithic column technology. Because of the high permeability of monolithic silica columns it should be possible to decrease the time for method development by applying high isocratic flow rates. Here we suggest a clear strategy for method development using monolithic columns. The strategy will be applicable for various sample compositions, e. g., acidic, basic, or neutral. The applicability of monolithic columns for especially complex separations of basic mixtures without the need of using a highly basic mobile phase that harms the column will be pointed out in this work. This work will describe in detail the actual method development process. For better understanding of our strategy, the influence of flow rate, column length, mobile phase composition, pH, and temperature will be discussed. Details about the application of a flow program will be mentioned.

A strategy to develop fast RP-HPLC methods using monolithic silica columns. Publishing Authors By Initials

S El Deeb,L Preu,H ,

For similar inorganic chemicals: minerals: silicon dioxide research abstracts see: inorganic chemicals: minerals: silicon dioxide research

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A strategy to develop fast RP-HPLC methods using monolithic silica columns. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of separation science

VOLUME: 30

Page Numbers: 1993-2001

Journal Abbreviation:

ISSN: 1615-9306

DAY: 28

MONTH: Aug

YEAR: 2007

A strategy to develop fast RP-HPLC methods using monolithic silica columns. Information

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LANGUAGE: eng

NlmUniqueID: 101088554

A strategy to develop fast RP-HPLC methods using monolithic silica columns. Keywords Mesh Terms:

KEYWORDS: Silicon Dioxide

MESH TERMS: chemistry

Chemical & Substance for Abstract: A strategy to develop fast RP-HPLC methods using monolithic silica columns. Information

Substance Name: Silicon Dioxide

Registry Number: 7631-86-9

Grant and Affiliation Information for A strategy to develop fast RP-HPLC methods using monolithic silica columns.

AFFILIATION: Institute of Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany.

Country: Germany

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MEDLINETA: J Sep Sci

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