A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA.

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Research Abstract Details 

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A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Abstract Text:

Brian A Sparano,Kazunori Koide,

We report a broadly applicable approach for the development of small-molecule-based RNA sensors. Our photoinduced electron transfer (PET) sensor consists of a fluorescein derivative as the fluorophore and two aniline derivatives as electron donors (quenchers). The isolation of electron-donor-binding RNA by in vitro selection (also known as SELEX) yielded an RNA aptamer that could increase the fluorescence intensity of the sensor by 13-fold. This result shows that RNA-electron-donor interactions can be used to develop modular RNA chemosensors.

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Publishing Authors By Initials

BA Sparano,K Koide,

For similar enzymes and coenzymes: enzymes: hydrolases: esterases: thiolester hydrolases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: esterases: thiolester hydrolases research

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A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 14954-5

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 2

MONTH: Nov

YEAR: 2005

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Keywords Mesh Terms:

KEYWORDS: Thiolester Hydrolases

MESH TERMS: metabolism

Chemical & Substance for Abstract: A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA. Information

Substance Name: Thiolester Hydrolases

Registry Number: EC 3.1.2.-

Grant and Affiliation Information for A strategy for the development of small-molecule-based sensors that strongly fluoresce when bound to a specific RNA.

AFFILIATION: Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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