A statistical method for chromatographic alignment of LC-MS data.

A statistical method for chromatographic alignment of LC-MS data. Research Abstract Details 

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A statistical method for chromatographic alignment of LC-MS data. Abstract Text:

Pei Wang,Hua Tang,Matthew P Fitzgibbon,Martin McIntosh,Marc Coram,Hui Zhang,Eugene Yi,Ruedi Aebersold,

Integrated liquid-chromatography mass-spectrometry (LC-MS) is becoming a widely used approach for quantifying the protein composition of complex samples. The output of the LC-MS system measures the intensity of a peptide with a specific mass-charge ratio and retention time. In the last few years, this technology has been used to compare complex biological samples across multiple conditions. One challenge for comparative proteomic profiling with LC-MS is to match corresponding peptide features from different experiments. In this paper, we propose a new method--Peptide Element Alignment (PETAL) that uses raw spectrum data and detected peak to simultaneously align features from multiple LC-MS experiments. PETAL creates spectrum elements, each of which represents the mass spectrum of a single peptide in a single scan. Peptides detected in different LC-MS data are aligned if they can be represented by the same elements. By considering each peptide separately, PETAL enjoys greater flexibility than time warping methods. While most existing methods process multiple data sets by sequentially aligning each data set to an arbitrarily chosen template data set, PETAL treats all experiments symmetrically and can analyze all experiments simultaneously. We illustrate the performance of PETAL on example data sets.

A statistical method for chromatographic alignment of LC-MS data. Publishing Authors By Initials

P Wang,H Tang,MP Fitzgibbon,M McIntosh,M Coram,H Zhang,E Yi,R Aebersold,

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A statistical method for chromatographic alignment of LC-MS data. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biostatistics (Oxford, England)

VOLUME: 8

Page Numbers: 357-67

Journal Abbreviation:

ISSN: 1465-4644

DAY: 31

MONTH: 07

YEAR: 2006

A statistical method for chromatographic alignment of LC-MS data. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100897327

A statistical method for chromatographic alignment of LC-MS data. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Electrospray Ionizat

MESH TERMS: methods

Chemical & Substance for Abstract: A statistical method for chromatographic alignment of LC-MS data. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for A statistical method for chromatographic alignment of LC-MS data.

AFFILIATION: Fred Hutchinson Cancer Research Center, Seattle, WA, USA. pwang@fhcrc.org

Country: England

AGENCY: United States NCI

GRANT: R21 CA114852

ACRONYM: CA

MEDLINETA: Biostatistics

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