A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice.

A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Research Abstract Details 

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A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Abstract Text:

A Takahashi,H Muramatsu,S Takagi,H Fujisawa,Y Miyake,T Muramatsu,

Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by only 3 amino acid residues from the human homologue. During mouse embryogenesis, Prp8 is expressed intensely at day 9.5 of gestation, and its expression decreases progressively during embryogenesis. In adult mice, Prp8 is expressed strongly in the testis and moderately in the ovary. in situ hybridization analysis revealed that Prp8 is preferentially expressed in the outer cell layer in the testis, probably in the spermatogonia and primary spermatocytes, and in granulosa cells in the ovary. In Caenorhabditis elegans, microinjection of a double stranded RNA corresponding to a portion of the Prp8 sequence results in the arrest of embryogenesis at the late-gastrulation stage. These results suggest that Prp8 plays an important role in reproduction and development. Prp8 was shown to bind to midkine (MK), a heparin-binding growth factor. Since Prp8 expression partially overlaps with the sites of action of MK, it is possible that binding to Prp8 is involved in part of MK signaling.

A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Publishing Authors By Initials

A Takahashi,H Muramatsu,S Takagi,H Fujisawa,Y Miyake,T Muramatsu,

For similar urogenital system: genitalia: genitalia, male: testis research abstracts see: urogenital system: genitalia: genitalia, male: testis research

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A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 129

Page Numbers: 599-606

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 2001

A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Keywords Mesh Terms:

KEYWORDS: Testis

MESH TERMS: embryology

Chemical & Substance for Abstract: A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice. Information

Substance Name: midkine

Registry Number: 137497-38-2

Grant and Affiliation Information for A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice.

AFFILIATION: Department of Biochemistry, Department of Ophthalmology, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

Country: Japan

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MEDLINETA: J Biochem

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ACCESSION NUMBER: AB047391

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