A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model.

A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Research Abstract Details 

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A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Abstract Text:

Kentaro Iwaki,Kohei Shibata,Masayuki Ohta,Yuichi Endo,Hiroki Uchida,Masayuki Tominaga,Ryoki Okunaga,Seiichiro Kai,Seigo Kitano,Kentaro Iwaki,Kohei Shibata,Masayuki Ohta,Yuichi Endo,Hiroki Uchida,Masayuki Tominaga,Ryoki Okunaga,Seiichiro Kai,Seigo Kitano,Kentaro Iwaki,Kohei Shibata,Masayuki Ohta,Yuichi Endo,Hiroki Uchida,Masayuki Tominaga,Ryoki Okunaga,Seiichiro Kai,Seigo Kitano,

Proteinase-activated receptor-2 (PAR-2), which is a G protein-coupled receptor, is activated in inflammatory processes and cell proliferation. We previously demonstrated that an anti-PAR-2 antibody suppresses proliferation of human pancreatic cells in vitro. However, there have been no studies of PAR-2 signaling pathways in vivo. The aim of this study was to determine whether blockade of PAR-2 by RNA interference influences pancreatic tumor growth. We originally constructed small interfering RNAs (siRNAs) targeting human PAR-2, and performed cell proliferation assays of Panc1 human pancreatic cancer cell line with these siRNAs. Intratumoral treatment with these PAR-2 siRNAs and atelocollagen was also performed in a xenograft model with nude mice and Panc1 cells. siRNAs against human PAR-2 inhibited proliferation of Panc1 cells, whereas control scramble siRNAs had no effect on proliferation. The PAR-2 siRNAs dramatically suppressed tumor growth in the xenograft model. PAR-2-specific siRNA inhibited growth of human pancreatic cancer cells both in vitro and in vivo. Blockade of PAR-2 signaling by siRNA may be a novel strategy to treat pancreatic cancer.

A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Publishing Authors By Initials

K Iwaki,K Shibata,M Ohta,Y Endo,H Uchida,M Tominaga,R Okunaga,S Kai,S Kitano,K Iwaki,K Shibata,M Ohta,Y Endo,H Uchida,M Tominaga,R Okunaga,S Kai,S Kitano,K Iwaki,K Shibata,M Ohta,Y Endo,H Uchida,M Tominaga,R Okunaga,S Kai,S Kitano,

For similar xenograft model antitumor assays research abstracts see: xenograft model antitumor assays research

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A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: International journal of cancer. Journal internati

VOLUME: 122

Page Numbers: 658-63

Journal Abbreviation: Int. J. Cancer

ISSN: 1097-0215

DAY: 1

MONTH: Feb

YEAR: 2008

A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Information

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LANGUAGE: eng

NlmUniqueID: 42124

A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Keywords Mesh Terms:

KEYWORDS: Xenograft Model Antitumor Assays

MESH TERMS: metabolism

Chemical & Substance for Abstract: A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model. Information

Substance Name: Receptor, PAR-2

Registry Number: 0

Grant and Affiliation Information for A small interfering RNA targeting proteinase-activated receptor-2 is effective in suppression of tumor growth in a Panc1 xenograft model.

AFFILIATION: Department of Surgery I, Oita University Faculty of Medicine, Oita, Japan. iwaki@ med.oita-u.ac.jp

Country: United States

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MEDLINETA: Int J Cancer

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