A site-directed mutagenesis study of yeast calmodulin.

A site-directed mutagenesis study of yeast calmodulin. Research Abstract Details 

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A site-directed mutagenesis study of yeast calmodulin. Abstract Text:

I Matsuura,K Ishihara,Y Nakai,M Yazawa,H Toda,K Yagi,

A site-directed mutagenesis study was carried out in order to understand the regulatory mechanism of calmodulin. We started from the yeast (Saccharomyces cerevisiae) calmodulin gene since it has many differences in amino acid sequence and inferior functional properties compared with the vertebrate calmodulin. Recombinant yeast calmodulins were generated in Escherichia coli transformed by constructed expression plasmids. Three recombinant calmodulins were obtained. The first two were YCM61G, in which the Ca2(+)-binding site 2 (the four Ca2(+)-binding EF-hand structures in calmodulin were numbered from the N-terminus) was converted to the same as that in vertebrate calmodulin, and YCM delta 132-148, in which the C-terminal half sequence of site 4 was deleted. These two recombinant calmodulins had the same maximum Ca2+ binding (3 mol/mol) as yeast calmodulin, which indicates that site 4 of yeast calmodulin was the one losing Ca2+ binding capacity. YCM delta 132-148 could not activate target enzymes, whereas its Ca2+ binding profile was similar to those of yeast calmodulin and YCM61G. Therefore, the structure in site 4 which cannot bind Ca2+ is indispensable for the regulatory function of yeast calmodulin. The complete regulatory function of vertebrate calmodulin can be attained by the combination of 4 Ca2+ binding structures. The negative charge cluster in the central alpha-helix region is suggested to stabilize the active conformation of calmodulin, since the third yeast calmodulin mutant, YCM83E, which had the negative charge cluster, increased the maximum activation of myosin light chain kinase.

A site-directed mutagenesis study of yeast calmodulin. Publishing Authors By Initials

I Matsuura,K Ishihara,Y Nakai,M Yazawa,H Toda,K Yagi,

For similar fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research abstracts see: fungi: ascomycota: saccharomycetales: saccharomyces: saccharomyces cerevisiae research

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A site-directed mutagenesis study of yeast calmodulin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 109

Page Numbers: 190-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1991

A site-directed mutagenesis study of yeast calmodulin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A site-directed mutagenesis study of yeast calmodulin. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae

MESH TERMS: genetics

Chemical & Substance for Abstract: A site-directed mutagenesis study of yeast calmodulin. Information

Substance Name: Myosin-Light-Chain Kinase

Registry Number: EC 2.7.1.117

Grant and Affiliation Information for A site-directed mutagenesis study of yeast calmodulin.

AFFILIATION: Department of Chemistry, Faculty of Science, Hokkaido University.

Country: JAPAN

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MEDLINETA: J Biochem

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