A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization.

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Abstract Text:

Vance P Lochridge,Michele E Hardy,Vance P Lochridge,Michele E Hardy,

Noroviruses cause epidemic outbreaks of acute viral gastroenteritis worldwide, and the number of reported outbreaks is increasing. Human norovirus strains do not grow in cell culture. However, murine norovirus (MNV) replicates in the RAW 264.7 macrophage cell line and thus provides a tractable model to investigate norovirus interactions with host cells. Epitopes recognized by monoclonal antibodies (MAbs) against the human norovirus strains Norwalk virus and Snow Mountain virus (SMV) identified regions in the P domain of major capsid protein VP1 important for interactions with putative cellular receptors. To determine if there was a relationship between domains of MNV VP1 and VP1 of human norovirus strains involved in cell binding, epitope mapping by phage display was performed with an MNV-1-neutralizing MAb, A6.2.1. A consensus peptide, GWWEDHGQL, was derived from 20 third-round phage clones. A synthetic peptide containing this sequence and constrained through a disulfide linkage reacted strongly with the A6.2.1 MAb, whereas the linear sequence did not. Four residues in the A6.2.1-selected peptide, G327, G333, Q334, and L335, aligned with amino acid residues in the P2 domain of MNV-1 VP1. This sequence is immediately adjacent to the epitope recognized by anti-SMV MAb 61.21. Neutralization escape mutants selected with MAb A6.2.1 contained a leucine-to-phenylalanine substitution at position 386 in the P2 domain. The predicted location of these residues on VP1 suggests that the phage peptide and the mutation in the neutralization-resistant viruses may be in close proximity to each other and to residues reported to be important for carbohydrate binding to VP1 of human norovirus strains.

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Publishing Authors By Initials

VP Lochridge,ME Hardy,VP Lochridge,ME Hardy,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of virology

VOLUME: 81

Page Numbers: 12316-22

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 5

MONTH: 09

YEAR: 2007

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization.

AFFILIATION: Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717-3610, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR020185

ACRONYM: RR

MEDLINETA: J Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization Related Publications

• A single-amino-acid substitution in the P2 domain of VP1 of murine norovirus is sufficient for escape from antibody neutralization.
• ABCA1 polymorphisms and Alzheimer's disease.
• Abnormal preantral folliculogenesis in polycystic ovaries is associated with increased granulosa cell division.
• Anterior cruciate ligament biology and its relationship to injury forces.
• Autoreactive B-1 B cells: Constraints on natural autoantibody B cell antigen receptors.
• B-1 B cells: development, selection, natural autoantibody and leukemia.
• Biphasic effects of postnatal exposure to diethylhexylphthalate on the timing of puberty in male rats.
• Breast cancer metastasis to bone: evaluation of bioluminescent imaging and microSPECT/CT for detecting bone metastasis in immunodeficient mice.
• Contributions of industry to parenteral nutrition.
• Correlation of an FE Model of the Human Head with Local Brain Motion--Consequences for Injury Prediction.
• Development of a cryopreservation protocol for Leydig cells.
• Encountering competitors reduces clutch size and increases offspring size in a parasitoid with female-female fighting.
• Giant prostatic utricle associated with proximal hypospadias: repair by mucosa divesting and muscular tunnel obliteration.
• Intravascular neutrophil activation due to carbon monoxide poisoning.
• Local planar gradients with order-of-magnitude strength and speed advantage.
• Measuring the impact of hepatitis A virus in Chinese cities: a life table approach.
• MedStar health vendor access program: one system's story.
• Respiratory tract infection with Mycoplasma pneumoniae in interleukin-12 knockout mice results in improved bacterial clearance and reduced pulmonary inflammation.
• Speed of lexical decision correlates with diffusion anisotropy in left parietal and frontal white matter: evidence from diffusion tensor imaging.
• Student-driven approach to addressing classroom disturbances suggested.
• Systemic cytokine profile in children with community-acquired pneumonia.
• Testosterone production in mice lacking inducible nitric oxide synthase expression is sensitive to restraint stress.
• The MAPT H1c risk haplotype is associated with increased expression of tau and especially of 4 repeat containing transcripts.
• The effect of different carbohydrate intakes on body weight and nitrogen balance during nasogastric feeding after faciomaxillary surgery.
• The metabolic syndrome: A high-risk state for cancer?
• The stability of ascorbic acid in TPN mixtures stored in a multilayered bag.
• Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability.
• [Double localization of an osteoid osteoma of the ankle: percutaneous treatment by CT-guided drilling]
• [Gender vulnerability for parenthood among male adolescents]
• [Relationship between phthalates and testicular dysgenesis syndrome]