A simple thermodynamic test to discriminate between two-state and downhill folding.

A simple thermodynamic test to discriminate between two-state and downhill folding. Research Abstract Details 

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A simple thermodynamic test to discriminate between two-state and downhill folding. Abstract Text:

Fabiana Y Oliva,Victor ,

The recent discovery of one-state folding, in which proteins unfold by progressive structural disorganizations (i.e., downhill folding), has emphasized the need for simple thermodynamic tests to discriminate between this behavior and classical two-state folding. On the basis of theoretical results from elementary statistical mechanical models, we propose such a test. The test involves monitoring the equilibrium unfolding transition induced by a combination of temperature and chemical denaturants with a probe that is sensitive to the average protein backbone conformation. The rationale is that the coupling between two different denaturation procedures can reveal subtle changes in protein conformational ensembles even when using bulk measurements. We demonstrate the applicability of the test by studying the unfolding process of the protein BBL, which has been previously characterized as a downhill folding protein. This test should be very useful for high-throughput design strategies and for the analysis of mutational effects in small proteins.

A simple thermodynamic test to discriminate between two-state and downhill folding. Publishing Authors By Initials

FY Oliva,V ,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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A simple thermodynamic test to discriminate between two-state and downhill folding. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 8596-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 21

MONTH: Jul

YEAR: 2004

A simple thermodynamic test to discriminate between two-state and downhill folding. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

A simple thermodynamic test to discriminate between two-state and downhill folding. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: chemistry

Chemical & Substance for Abstract: A simple thermodynamic test to discriminate between two-state and downhill folding. Information

Substance Name: Urea

Registry Number: 57-13-6

Grant and Affiliation Information for A simple thermodynamic test to discriminate between two-state and downhill folding.

AFFILIATION: Department of Chemistry and Biochemistry and Center for Biomolecular Structure and Organization, University of Maryland, College Park, Maryland 20742, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM066800-01

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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