A simple tagging system for protein encapsulation.

A simple tagging system for protein encapsulation. Research Abstract Details 

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A simple tagging system for protein encapsulation. Abstract Text:

Florian P Seebeck,Kenneth J Woycechowsky,Wei Zhuang, Rabe,Donald Hilvert,Florian P Seebeck,Kenneth J Woycechowsky,Wei Zhuang, Rabe,Donald Hilvert,

Molecular containers that encapsulate specific cargo can be useful for many natural and non-natural processes. We report a simple system, based on charge complementarity, for the encapsulation of appropriately tagged proteins within an engineered, proteinaceous capsid. Four negative charges per monomer were added to the lumazine synthase from Aquifex aeolicus (AaLS). The capsids formed by the engineered AaLS associate with green fluorescent protein bearing a positively charged deca-arginine tag upon coproduction in Escherichia coli. Analytical ultracentrifugation and scanning force microscopy studies indicated that the engineered AaLS retains the ability to form capsids, but that their average size was substantially increased. The success of this strategy demonstrates that both the container and guest components of protein-based encapsulation systems can be convergently designed in a straightforward manner, which may help to extend their versatility.

A simple tagging system for protein encapsulation. Publishing Authors By Initials

FP Seebeck,KJ Woycechowsky,W Zhuang,JP Rabe,D Hilvert,FP Seebeck,KJ Woycechowsky,W Zhuang,JP Rabe,D Hilvert,

For similar investigative techniques: genetic techniques: genetic engineering: protein engineering research abstracts see: investigative techniques: genetic techniques: genetic engineering: protein engineering research

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MEDLINE DATE:

A simple tagging system for protein encapsulation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 4516-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 12

MONTH: Apr

YEAR: 2006

A simple tagging system for protein encapsulation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

A simple tagging system for protein encapsulation. Keywords Mesh Terms:

KEYWORDS: Protein Engineering

MESH TERMS: genetics

Chemical & Substance for Abstract: A simple tagging system for protein encapsulation. Information

Substance Name: 6,7-dimethyl-8-ribityllumazine synthase

Registry Number: 89287-46-7

Grant and Affiliation Information for A simple tagging system for protein encapsulation.

AFFILIATION: Laboratorium für Organische Chemie, ETH Zürich, Hönggerberg HCI F337, CH-8093, Zürich, Switzerland.

Country: United States

AGENCY: United States NIGMS

GRANT: 1 F32 GM071126-01A1

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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