A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography.

A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Research Abstract Details 

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A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Abstract Text:

Using immobilized monoclonal antibodies (anti-HBs) to hepatitis B surface antigen (HBs) as an immunosorbent affinity column, a simple and effective procedure for HBs purification has been developed. A serum sample containing high titer HBs (i.e. HBe-positive serum) is passed through the column without prior treatment. The HBs is further purified by cesium chloride gradient ultracentrifugation. The recovery of HBs is greater than 70% while the purity is very comparable to those obtained from several isopycnic and rate zonal ultracentrifugation procedures (Dreesman et al., 1972). Over a period of two months, the column was used repeatedly for thirty cycles without any noticeable deterioration.

A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Publishing Authors By Initials

For similar natural sciences: chemistry: chemistry, physical: molecular weight research abstracts see: natural sciences: chemistry: chemistry, physical: molecular weight research

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A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Science Council, Repub

VOLUME: 10

Page Numbers: 86-91

Journal Abbreviation: Proc. Natl. Sci. Counc. Repub.

ISSN: 0255-6596

DAY: 26

MONTH: Apr

YEAR: 1986

A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 8502426

A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Keywords Mesh Terms:

KEYWORDS: Molecular Weight

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography. Information

Substance Name: Hepatitis B Surface Antigens

Registry Number: 0

Grant and Affiliation Information for A simple purification procedure for hepatitis B surface antigen (HBs) by monoclonal antibody immunosorbent affinity chromatography.

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Country: TAIWAN

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MEDLINETA: Proc Natl Sci Counc Repub Chin

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