A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues.

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Abstract Text:

Jeffrey G Jacot,Scott Dianis,Joshua Schnall,Joyce Y Wong,

Several recent studies have shown that cells respond to the elastic modulus and elasticity gradients on soft substrates. However, traditional macroscale methods for measuring elastic modulus cannot resolve elastic gradients or differences between the macroscale and microscale elastic modulus of layered tissues. Here, we present a technique for measurement of the microscale elastic modulus of soft, hydrated gels and tissues. This technique requires less equipment than equivalent atomic force microscopy (AFM) and can easily measure larger samples with high adhesiveness. We validate this technique by measuring the microscale modulus of a hydrogel with elasticity that does not depend on measurement scale. We show that the elastic modulus measured using microindentation correlates with measurements using AFM and the macroscale tensile modulus. We verified the ability of this technique to characterize a hydrogel with an elastic gradient of 2.2 kPa/mm across 19 mm and to measure the microscale elastic modulus of the endothelial side of human greater saphenous vein, which is an order of magnitude less than the whole vein macroscale modulus. This simple, inexpensive system allows the measurement of the spatial organization of microscale elastic properties of fully hydrated, soft gels and tissues as a routine laboratory technique.

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Publishing Authors By Initials

JG Jacot,S Dianis,J Schnall,JY Wong,

For similar water research abstracts see: water research

PUBMED ID PMID:

MEDLINE DATE:

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biomedical materials research. Part A

VOLUME: 79

Page Numbers: 485-94

Journal Abbreviation:

ISSN: 1549-3296

DAY: 1

MONTH: Dec

YEAR: 2006

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Keywords Mesh Terms:

KEYWORDS: Water

MESH TERMS: chemistry

Chemical & Substance for Abstract: A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues. Information

Substance Name: polyacrylamide

Registry Number: 9003-05-8

Grant and Affiliation Information for A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues.

AFFILIATION: Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL72900-01

ACRONYM: HL

MEDLINETA: J Biomed Mater Res A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues Related Publications

• A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues.
• A study of density of states and ground states in hydrophobic-hydrophilic protein folding models by equi-energy sampling.
• An extended simplified reference tissue model for the quantification of dynamic PET with amphetamine challenge.
• Cell cycle dysregulation in oral cancer.
• Characterization of a transglycosylase domain of Streptococcus pneumoniae PBP1b.
• Differentially expressed protein markers in human submandibular and sublingual secretions.
• Dose effects of triazolam on brain activity during episodic memory encoding: a PET study.
• Driver perception response time during the signal change interval.
• Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo.
• Extracellular heat shock protein-70 induces endotoxin tolerance in THP-1 cells.
• Feruloyl esterase: a key enzyme in biomass degradation.
• German cockroach proteases regulate matrix metalloproteinase-9 in human bronchial epithelial cells.
• HIV Evolution and Escape.
• Health services utilization for people with HIV infection: comparison of a population targeted for outreach with the U.S. population in care.
• In vivo imaging reveals dendritic targeting of laminated afferents by zebrafish retinal ganglion cells.
• In vivo optical coherence tomography of the nasal mucosa.
• Measurement of serum D-arabinitol/creatinine ratios for initial diagnosis and for predicting outcome in an unselected, population-based sample of patients with Candida fungemia.
• Mechanism and function of heat shock-dependent IkappaBalpha expression.
• Mycotic aortic aneurysm following treatment of pyogenic vertebral osteomyelitis.
• Prediction of breast cancer using volatile biomarkers in the breath.
• Prolonged and severe dysphagia following anterior cervical surgery.
• Sugar-assisted ligation of N-linked glycopeptides with broad sequence tolerance at the ligation junction.
• The executive clock drawing task (CLOX) is a poor screening test for executive dysfunction in Chinese elderly patients with subcortical ischemic vascular disease.
• The relationship between parent emotion, parent behavior, and health status of young African American and Latino children.
• [Advances of fine needle aspiration cytology]
• [Biosynthesis and characterization of jarosite]
• [Creation of the World Federation of Critical Care Nursing (WFCCN)]
• [Effect of oxygen on the fluorescence of sol-gel with ruthenium complexes]
• [In Process Citation]
• c-Jun NH(2)-terminal kinase 2alpha2 promotes the tumorigenicity of human glioblastoma cells.