A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate.

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Abstract Text:

T Kinoshita,K Kanada,A Tsuji,

A simple chemical method for the determination of individual mucopolysaccharides in mixtures of dermatan sulfate and chondroitin sulfates by means of a single reagent was established, utilizing the difference in reaction rates of these polysaccharides with orcinol. To each 1 ml of a sample mixture of standard dermatan sulfate and standard chondroitin sulfate (either 4- or 6-sulfate) was added 3 ml of orcinol reagent and the resulting solution was heated in a boiling-water bath. After 20 and 60 min reaction, absorbances at 660 nm were measured and the concentrations of individual mucopolysaccharides were calculated. High reproducibility was observed for the determination of dermatan sulfate in the presence of chondroitin sulfates. In addition, orcinol reaction for 90 min employing D-glucuronolactone as a standard appeared to be of practical value in the estimation of the uronic acid content of these mucopolysaccharides.

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Publishing Authors By Initials

T Kinoshita,K Kanada,A Tsuji,

For similar organic chemicals: carboxylic acids: acids, acyclic: sugar acids: uronic acids research abstracts see: organic chemicals: carboxylic acids: acids, acyclic: sugar acids: uronic acids research

PUBMED ID PMID:

MEDLINE DATE:

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 77

Page Numbers: 399-403

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1975

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Keywords Mesh Terms:

KEYWORDS: Uronic Acids

MESH TERMS: analysis

Chemical & Substance for Abstract: A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate. Information

Substance Name: Chondroitin

Registry Number: 9007-27-6

Grant and Affiliation Information for A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate Related Publications

• A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate.
• Alcohol consumption and suicide mortality among Japanese men: the Ohsaki Study.
• Chicken ornithine transcarbamylase: purification and some properties.
• Cilnidipine lowered psychological stress-induced increase in blood pressure in a hypertensive man: a case report.
• Comparative analysis of the genomic structures and promoter activities of mouse Siaalpha2,3Galbeta1,3GalNAc GalNAcalpha2, 6-sialyltransferase genes (ST6GalNAc III and IV): characterization of their Sp1 binding sites.
• Cycling of an 85-kDa lysosomal membrane glycoprotein between the cell surface and lysosomes in cultured rat hepatocytes.
• Cycling of two endogenous lysosomal membrane proteins, lamp-2 and acid phosphatase, between the cell surface and lysosomes in cultured rat hepatocytes.
• Fucosylated Thomsen-Friedenreich antigen in alpha-anomeric configuration in human gastric surface epithelia: an allogeneic carbohydrate antigen possibly controlled by the Se gene.
• Function of NKT cells, potential anti-HIV effector cells, are improved by beginning HAART during acute HIV-1 infection.
• Genomic organization and transcriptional regulation of the mouse GD3 synthase gene (ST8Sia I): comparison of genomic organization of the mouse sialyltransferase genes.
• Involvement of organic anion transport system in transdermal absorption of flurbiprofen.
• Molecular cloning and genomic analysis of mouse GalNAc alpha2, 6-sialyltransferase (ST6GalNAc I).
• Molecular survey of Babesia microti, Ehrlichia species and Candidatus neoehrlichia mikurensis in wild rodents from Shimane Prefecture, Japan.
• Nanostructured bulk copper fabricated by accumulative roll bonding.
• PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element.
• Phosphatidylserine specific binding protein in rat brain: purification and characterization.
• Poly(lactide) Stereocomplexes: Formation, Structure, Properties, Degradation, and Applications.
• Purification and characterization of a major kyotorphin-hydrolyzing peptidase of rat brain.
• Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver.
• Species-specific epitopes exist on the cytoplasmic amino-terminal domain of erythrocyte band 3 protein.
• The Outcome of Gastrointestinal Stromal Tumors (GISTs) After a Surgical Resection in Our Institute.
• The Thomsen-Friedenreich antigen-related carbohydrate antigens in human gastric intestinal metaplasia and cancer.
• Transcriptional regulation of subtilisin-like proprotein convertase PACE4 by E2F: possible role of E2F-mediated upregulation of PACE4 in tumor progression.
• Transient expression of a brain/embryonic-type myosin heavy chain isoform (MIIB2) in regenerating rat liver.
• Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa.
• Transporter-mediated hepatic uptake of ulifloxacin, an active metabolite of a prodrug-type new quinolone antibiotic prulifloxacin, in rats.
• Unique mechanical properties of nanostructured metals.
• [Occupation and carpal tunnel syndrome]
• [Skin reaction induced by subcutaneous injection of LH-RH analogue]
• [Splenic marginal zone lymphoma associated with autoimmune hemolytic anemia treated with splenectomy and rituximab]