A simple and rapid method for cryopreservation of Trichomonas vaginalis.

A simple and rapid method for cryopreservation of Trichomonas vaginalis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A simple and rapid method for cryopreservation of Trichomonas vaginalis. Abstract Text:

Junji Matsuo,Junji Matsuo,Junji Matsuo,

A pellet freezing method, one type of rapid freezing protocol, was established for Trichomonas vaginalis. As cryoprotectants, 5-20% dimethylsulfoxide (DMSO), ethylene glycol, propylene glycol, and glycerol were used. Of these, DMSO showed a high protective effect, with a maximum effect at a concentration of 20%. No effect of equilibration time was recognized with any of the cryoprotectants. Therefore, the optimal condition was deemed 20% DMSO without equilibration. The pellet freezing method was also compared with the conventional slow cooling protocol. The survival rate was 73 +/- 8% with the slow cooling protocol and 48 +/- 4% with the pellet freezing method. The delay in growth with the pellet freezing method was no more than 5.4 h compared with the slow cooling protocol, suggesting its validity as a technique for cryopreservation of T. vaginalis.

A simple and rapid method for cryopreservation of Trichomonas vaginalis. Publishing Authors By Initials

J Matsuo,J Matsuo,J Matsuo,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A simple and rapid method for cryopreservation of Trichomonas vaginalis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Parasitology research

VOLUME: 101

Page Numbers: 907-11

Journal Abbreviation: Parasitol. Res.

ISSN: 0932-0113

DAY: 13

MONTH: 05

YEAR: 2007

A simple and rapid method for cryopreservation of Trichomonas vaginalis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8703571

A simple and rapid method for cryopreservation of Trichomonas vaginalis. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A simple and rapid method for cryopreservation of Trichomonas vaginalis. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A simple and rapid method for cryopreservation of Trichomonas vaginalis.

AFFILIATION: Division of Medical Technology, Department of Health Sciences, Hokkaido University School of Medicine, North-12, West-5, Kita-ku, Sapporo, 060-0812, Japan, matsuo@cme.hokudai.ac.jp.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Parasitol Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A simple and rapid method for cryopreservation of Trichomonas vaginalis Related Publications

• A simple and rapid method for cryopreservation of Trichomonas vaginalis.
• Author reply to recent improvement in the survival of patients with advanced nonsmall cell lung cancer enrolled in phase III trials of first-line, systemic chemotherapy.
• Caries-related Bacteria and Cytokines Induce CXCL10 in Dental Pulp.
• Comparison of Prevalence Rates of Strabismus and Amblyopia in Japanese Elementary School Children between the Years 2003 and 2005.
• Cooperative effects of daunorubicin and actinomycin D on the sporulation of Bacillus subtilis.
• Development of a multi-Fourier-transform interferometer: imaging experiments in millimeter and submillimeter wave bands.
• Effectiveness of narrow-band ultraviolet-B phototherapy for prevention of intimal hyperplasia in a rat carotid balloon injury model.
• Flagella facilitate escape of Salmonella from oncotic macrophages.
• Free N-glycans already occur at an early stage of seed development.
• Improvement in Discomfort and Decreased Blood Flow Associated with Severe Arterial Wall Calcification following Etidronic Acid.
• Isolation and determination of the amino acid sequence of chicken calcitonin I from chicken ultimobranchial glands.
• Kidney disease screening program in Japan: history, outcome, and perspectives.
• Modification of the Modification of Diet in Renal Disease (MDRD) Study equation for Japan.
• Mosaic tetrasomy 9p case with the phenotype mimicking klinefelter syndrome and hyporesponse of gonadotropin-stimulated testosterone production.
• Multivariate analysis of the factors associated with the risk of pneumonia in intensive care units.
• Neural mechanism and possible role of inhibition of gastric motility induced by superior laryngeal afferents.
• Purification and characterization of [3H]mepyramine (histamine H1 antagonist)-binding protein from rat liver: a highly homologous protein with cytochrome P450 2D.
• Purification and characterization of a peptide C-terminal alpha-amidating enzyme from porcine atrium.
• Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis.
• Salivary secretion, taste and hyposalivation.
• Sphingomyelin-dependence of cholesterol efflux mediated by ABCG1.
• The JICA training course, community-based cancer prevention for the Asian and Pan-Pacific countries, fiscal year 2006 (epidemiological approach).
• Toxicity of cyclic compound of beta-eleostearic acid.
• [A case of Menkes disease effectively treated with tocopherol acetate]
• [CKD (chronic kidney disease)]
• [Drug induced nephropathy]
• [Health care and mortality of very-low-birth-weight neonates.]
• [Odorant-binding proteins and evolution of host-plant preference in insects]
• [Prevalence and characterization of chronic pain among the elderly living in the community]
• [Safe drug prescribing for patients with renal failure]