An air-drying method for chromosome preparation was improved for use in zona-penetrated mouse embryos. This method consisted of a sequence of short-term (1 to 3 min) hypotonic treatments, a two-step fixation process and slow air-drying under heavy moisture. The method is also applicable to intact embryos at the one-cell, morula, and blastocyst stages for chromosome analysis of embryos at each of these stages.
A short-term hypotonic treatment for chromosome preparation of intact and zona-penetrated mouse embryos. Publishing Authors By Initials
A short-term hypotonic treatment for chromosome preparation of intact and zona-penetrated mouse embryos. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Theriogenology
VOLUME: 33
Page Numbers: 789-97
Journal Abbreviation: Theriogenology
ISSN: 0093-691X
DAY: 26
MONTH: Apr
YEAR: 1990
A short-term hypotonic treatment for chromosome preparation of intact and zona-penetrated mouse embryos. Information
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LANGUAGE: eng
NlmUniqueID: 421510
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Grant and Affiliation Information for A short-term hypotonic treatment for chromosome preparation of intact and zona-penetrated mouse embryos.
AFFILIATION: Department of Animal Breeding & Reproduction, Faculty of Agriculture University of Utsunomiya, Mine-machi 350, Utsunomiya 321, Japan.
Country: United States
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MEDLINETA: Theriogenology
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