A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization.

A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Research Abstract Details 

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A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Abstract Text:

Joseph LeSauter,Lily Yan,Bhavana Vishnubhotla,Jorge E Quintero,Sandra J Kuhlman,Douglas G McMahon,Rae Silver,

Period1 (Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We examined a transgenic mouse in which degradable green fluorescent protein (GFP) is driven by the mPer1 gene promoter. This mouse expresses precise free-running rhythms and characteristic light induced phase shifts. GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted rhythms of Per1 mRNA, PER1 and PER2 proteins. The localization of GFP overlaps with that of Per1 mRNA, PER1 and PER2 proteins. Together, these results suggest that GFP reports rhythmic Per1 expression. A surprising finding is that, at their peak expression time GFP, Per1 mRNA, PER1 and PER2 proteins are absent or not detectable in a subpopulation of SCN cells located in the core region of the nucleus.

A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Publishing Authors By Initials

J LeSauter,L Yan,B Vishnubhotla,JE Quintero,SJ Kuhlman,DG McMahon,R Silver,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Brain research

VOLUME: 964

Page Numbers: 279-87

Journal Abbreviation: Brain Res.

ISSN: 0006-8993

DAY: 28

MONTH: Feb

YEAR: 2003

A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Information

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LANGUAGE: eng

NlmUniqueID: 45503

A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization.

AFFILIATION: Department of Psychology, Barnard College, 3009 Broadway, New York, NY 10027, USA.

Country: Netherlands

AGENCY: United States NINDS

GRANT: NS-37919

ACRONYM: NS

MEDLINETA: Brain Res

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