A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF.

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Abstract Text:

Influenza viruses are classified into three types: A, B, and C. The genomes of A- and B-type influenza viruses consist of eight RNA segments, whereas influenza C viruses only have seven RNAs. Both A and B influenza viruses contain two major surface glycoproteins: the hemagglutinin (HA) and the neuraminidase (NA). Influenza C viruses have only one major surface glycoprotein, HEF (hemagglutinin-esterase fusion). By using reverse genetics, we generated two seven-segmented chimeric influenza viruses. Each possesses six RNA segments from influenza virus A/Puerto Rico/8/34 (PB2, PB1, PA, NP, M, and NS); the seventh RNA segment encodes either the influenza virus C/Johannesburg/1/66 HEF full-length protein or a chimeric protein HEF-Ecto, which consists of the HEF ectodomain and the HA transmembrane and cytoplasmic regions. To facilitate packaging of the heterologous segment, both the HEF and HEF-Ecto coding regions are flanked by HA packaging sequences. When introduced as an eighth segment with the NA packaging sequences, both viruses are able to stably express a green fluorescent protein (GFP) gene, indicating a potential use for these viruses as vaccine vectors to carry foreign antigens. Finally, we show that incorporation of a GFP RNA segment enhances the growth of seven-segmented viruses, indicating that efficient influenza A viral RNA packaging requires the presence of eight RNA segments. These results support a selective mechanism of viral RNA recruitment to the budding site.

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 82

Page Numbers: 6419-26

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 30

MONTH: 04

YEAR: 2008

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF.

AFFILIATION: Department of Microbiology, Mount Sinai School of Medicine, Box 1124, One Gustave L. Levy Place, New York, NY 10029, USA.

Country: United States

AGENCY: United States NIAID

GRANT: UO1AI070469

ACRONYM: AI

MEDLINETA: J Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF Related Publications

• A combination in-ovo vaccine for avian influenza virus and Newcastle disease virus.
• A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF.
• A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 influenza A viruses contributes to increased virulence.
• Cellular proteins in influenza virus particles.
• Editorial comment.
• Effective replication of human influenza viruses in mice lacking a major alpha2,6 sialyltransferase.
• Expression of transgenes from newcastle disease virus with a segmented genome.
• High Temperature (30{degrees}C) Blocks Aerosol but Not Contact Transmission of Influenza Virus.
• Highly conserved regions of influenza a virus polymerase gene segments are critical for efficient viral RNA packaging.
• Human genes and influenza.
• Influenza A virus PB1-F2: a small protein with a big punch.
• Influenza B Virus NS1-Truncated Mutants: Live-Attenuated Vaccine Approach.
• Influenza virus transmission is dependent on relative humidity and temperature.
• Influenza virus transmission: basic science and implications for the use of antiviral drugs during a pandemic.
• Noninvasive adrenal imaging in hyperaldosteronism.
• Oseltamivir-resistant influenza A viruses are transmitted efficiently among guinea pigs by direct contact but not by aerosol.
• Protective immunity and susceptibility to infectious diseases: lessons from the 1918 influenza pandemic.
• Severe acute respiratory syndrome coronavirus open reading frame (ORF) 3b, ORF 6, and nucleocapsid proteins function as interferon antagonists.
• Specific residues of the influenza A virus hemagglutinin viral RNA are important for efficient packaging into budding virions.
• The effectiveness of continuing education in postoperative pain management: results from a follow-up study.