A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake.

A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Research Abstract Details 

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A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Abstract Text:

T Ueno,T Sekine,

To ascertain the function of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles, the effect of pH gradient on Ca2+ transport was examined. A transient H+ gradient (inside-acidic) was imposed on K+-loaded sarcoplasmic reticulum vesicles with the aid of K+-H+ exchange driven by nigericin. This proton gradient was dissipated rapidly and concomitantly with ATP-driven Ca2+ transport. Under these conditions, the initial rate of the Ca2+ uptake was increased about 1.5-fold. The stimulation of Ca2+ uptake was completely lost when the pH gradient was cancelled with an uncoupler plus membrane permeable cation before Ca2+ uptake. These results are interpreted in terms of H+ efflux coupled with Ca2+ transport.

A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Publishing Authors By Initials

T Ueno,T Sekine,

For similar polycyclic compounds: macrocyclic compounds: peptides, cyclic: depsipeptides: valinomycin research abstracts see: polycyclic compounds: macrocyclic compounds: peptides, cyclic: depsipeptides: valinomycin research

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A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 89

Page Numbers: 1239-46

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1981

A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Information

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LANGUAGE: eng

NlmUniqueID: 376600

A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Keywords Mesh Terms:

KEYWORDS: Valinomycin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: A role of H+ flux in active Ca2+ transport into sarcoplasmic reticulum vesicles. I. Effect of an artificially imposed H+ gradient on Ca2+ uptake. Information

Substance Name: Calcium

Registry Number: 7440-70-2

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Country: JAPAN

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MEDLINETA: J Biochem

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