A role for clathrin in reassembly of the Golgi apparatus.

A role for clathrin in reassembly of the Golgi apparatus. Research Abstract Details 

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A role for clathrin in reassembly of the Golgi apparatus. Abstract Text:

Andreea E Radulescu,Anirban Siddhanta,Dennis Shields,

The Golgi apparatus is a highly dynamic organelle whose organization is maintained by a proteinaceous matrix, cytoskeletal components, and inositol phospholipids. In mammalian cells, disassembly of the organelle occurs reversibly at the onset of mitosis and irreversibly during apoptosis. Several pharmacological agents including nocodazole, brefeldin A (BFA), and primary alcohols (1-butanol) induce reversible fragmentation of the Golgi apparatus. To dissect the mechanism of Golgi reassembly, rat NRK and GH3 cells were treated with 1-butanol, BFA, or nocodazole. During washout of 1-butanol, clathrin, a ubiquitous coat protein implicated in vesicle traffic at the trans-Golgi network and plasma membrane, and abundant clathrin coated vesicles were recruited to the region of nascent Golgi cisternae. Knockdown of endogenous clathrin heavy chain showed that the Golgi apparatus failed to reform efficiently after BFA or 1-butanol removal. Instead, upon 1-butanol washout, it maintained a compact, tight morphology. Our results suggest that clathrin is required to reassemble fragmented Golgi elements. In addition, we show that after butanol treatment the Golgi apparatus reforms via an initial compact intermediate structure that is subsequently remodeled into the characteristic interphase lace-like morphology and that reassembly requires clathrin.

A role for clathrin in reassembly of the Golgi apparatus. Publishing Authors By Initials

AE Radulescu,A Siddhanta,D Shields,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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A role for clathrin in reassembly of the Golgi apparatus. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 94-105

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 25

MONTH: 10

YEAR: 2006

A role for clathrin in reassembly of the Golgi apparatus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

A role for clathrin in reassembly of the Golgi apparatus. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: drug effects

Chemical & Substance for Abstract: A role for clathrin in reassembly of the Golgi apparatus. Information

Substance Name: 1-Butanol

Registry Number: 71-36-3

Grant and Affiliation Information for A role for clathrin in reassembly of the Golgi apparatus.

AFFILIATION: Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32GM-07491

ACRONYM: GM

MEDLINETA: Mol Biol Cell

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